Evidence map›Paper›PMID 30332749›Full record

ArticleInternational journal of molecular sciences2018

Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons.

Daniel Andersson, David Svec, Cathrine Pedersen, Jørn Remi Henriksen, Anders Ståhlberg

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Rapid and highly sensitive approach for multiplexed somatic fusion detection.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2022
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 3 countries.

Daniel AnderssonSahlgrenska Cancer Center, Department of Pathology and Genetics, Sahlgrenska Academy at University of Gothenburg, Box 425, 40530 Gothenburg, Sweden. daniel.andersson.3@gu.se.ORCID 0000-0002-4564-6652
David SvecSahlgrenska Cancer Center, Department of Pathology and Genetics, Sahlgrenska Academy at University of Gothenburg, Box 425, 40530 Gothenburg, Sweden. david.svec@ibt.cas.cz.ORCID 0000-0002-3893-6803
Cathrine PedersenArcticZymes AS, Sykehusveien 23, 9019 Tromsø, Norway. cap@arcticzymes.com.
Jørn Remi HenriksenArcticZymes AS, Sykehusveien 23, 9019 Tromsø, Norway. jrh@arcticzymes.com.
Anders StåhlbergSahlgrenska Cancer Center, Department of Pathology and Genetics, Sahlgrenska Academy at University of Gothenburg, Box 425, 40530 Gothenburg, Sweden. anders.stahlberg@gu.se.ORCID 0000-0003-4243-0191
Sahlgrenska University Hospital · SEArcticZymes (Norway) · NO

Funding

Barncancerfonden PR2015-0076Barncancerfonden TJ2015-0058BioCARE National Strategic Research Program at University of Gothenburg -Cancerfonden CAN 2016/438European Regional Development Fund BIOCEV: CZ.1.05/2.1.00/19.0390Grantová Agentura České Republiky 18-24753YJohan Jansson Foundation for Cancer Research -Knut and Alice Wallenberg Foundation, Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Sweden -Norges Forskningsråd 235264RVO 86652036Sahlgrenska Academy (ALF) at University of Gothenburg ALFGBG-716321Sahlgrenska Universitetssjukhusets stiftelser -Stiftelsen Anna-Brita och Bo Castegrens Minne -Stiftelsen Assar Gabrielssons Fond -Stiftelserna Wilhelm och Martina Lundgrens -Svenska Sällskapet för Medicinsk Forskning -Vetenskapsrådet 2017-01392VINNOVA -
6 · The paper itself

Abstract

Analyzing rare DNA and RNA molecules in limited sample sizes, such as liquid biopsies and single cells, often requires preamplification, which makes downstream analyses particularly sensitive to polymerase chain reaction (PCR) generated contamination. Herein, we assessed the feasibility of performing Cod uracil-DNA

Indexed as

DNA ContaminationAnimalsDeoxyuracil NucleotidesGadus morhuaGene Expression ProfilingReproducibility of ResultsSingle-Cell AnalysisUracilUracil-DNA GlycosidaseDeoxyuracil Nucleotidesdeoxyuridine triphosphateUracilUracil-DNA GlycosidaseCod UNGcontaminationdUTPpreamplificationqPCRsingle-cell analysis

Identifiers

PMID30332749
PMCPMC6214100
OpenAlexW2896631609

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.