ArticleCell reports2018
Insights into Bidirectional Gene Expression Control Using the Canonical GAL1/GAL10 Promoter.
Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Analysis of the PRE configuration reveals the constraints in Ste12 oligomerization in mediating pheromone-inducible transcription inEngineering microbiology · 2026Article
- Rapid Screening and Effective Rabbit-Derived Fab Antibodies Production Based on Yeast Surface Display.Combinatorial chemistry & high throughput screening · 2026Article
- Unraveling the regulatory dynamics of bidirectional promoters for modulating gene co-expression and metabolic flux in Saccharomyces cerevisiae.Nucleic acids research · 2025Article
- Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.Nature communications · 2024Article
- A highly selective cell-based fluorescent biosensor for genistein detection.Engineering microbiology · 2023Article
- Transcription-replication coordination revealed in single live cells.Nucleic acids research · 2022Article
- Production of galactosylated complex-type N-glycans in glycoengineered Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2022Article
- Phenotypic selection during laboratory evolution of yeast populations leads to a genome-wide sustainable chromatin compaction shift.Frontiers in microbiology · 2022Article
- Phenotypic plasticity as a facilitator of microbial evolution.Environmental epigenetics · 2022Review
- A synthetic promoter system for well-controlled protein expression with different carbon sources in Saccharomyces cerevisiae.Microbial cell factories · 2021Article
- Decoupling transcription factor expression and activity enables dimmer switch gene regulation.Science (New York, N.Y.) · 2021Article
- Chromatin regulatory genes differentially interact in networks to facilitate distinct GAL1 activity and noise profiles.Current genetics · 2021Article
- Functional and transcriptional profiling of non-coding RNAs in yeast reveal context-dependent phenotypes and in trans effects on the protein regulatory network.PLoS genetics · 2021Article
- Genome Editing in Cereals: Approaches, Applications and Challenges.International journal of molecular sciences · 2020Review
- Functional characterization of an inducible bidirectional promoter from Fusarium oxysporum f. sp. cubense.Scientific reports · 2020Article
- Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing.Frontiers in plant science · 2019Article
- Simultaneous co-overexpression of Saccharomyces cerevisiae septins Cdc3 and Cdc10 drives pervasive, phospholipid-, and tag-dependent plasma membrane localization.Cytoskeleton (Hoboken, N.J.)Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Despite advances made in understanding the effects of promoter structure on transcriptional activity, limited knowledge exists regarding the role played by chromatin architecture in transcription. Previous work hypothesized that transcription from the bidirectional GAL1/GAL10 promoter is controlled through looping of its UAS region around a nonstandard nucleosome. Here, by editing the GAL1/GAL10 promoter at high resolution, we provide insights into bidirectional expression control. We demonstrate that the first and fourth Gal4 binding sites within the UAS do not functionally contribute to promoter activation. Instead, these sites, along with nearby regulatory regions, contribute to the directional regulation of gene expression. Furthermore, Gal4 binding to the third binding site is critical for gene expression, while binding to the other three sites is not sufficient for transcriptional activation. Because the GAL1/GAL10 UAS can activate gene expression in many eukaryotes, the regulatory mechanism presented is expected to operate broadly across the eukaryotic clade.
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