ArticleChembiochem : a European journal of chemical biology2018
NAIL-MS in E. coli Determines the Source and Fate of Methylation in tRNA.
Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- tRNA Modification Landscapes in Streptococci: Shared Losses and Clade-Specific Adaptations.bioRxiv : the preprint server for biology · 2025Article
- Protective effect and possible mechanism of arctiin on broilers challenged by Salmonella pullorum.Journal of animal science · 2022Article
- The Stress-Dependent Dynamics ofGenes · 2021Article
- Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo.Nature communications · 2021Article
- Article
- Synthesis and Metabolic Fate of 4-Methylthiouridine in Bacterial tRNA.Chembiochem : a European journal of chemical biology · 2020Article
- Review
- Broadly applicable oligonucleotide mass spectrometry for the analysis of RNA writers and erasers in vitro.Nucleic acids research · 2020Article
- Identification of the 3-amino-3-carboxypropyl (acp) transferase enzyme responsible for acp3U formation at position 47 in Escherichia coli tRNAs.Nucleic acids research · 2020Article
- NAIL-MS reveals the repair of 2-methylthiocytidine by AlkB in E. coli.Nature communications · 2019Article
- To be or not to be modified: Miscellaneous aspects influencing nucleotide modifications in tRNAs.IUBMB life · 2019Review
- Production and Application of Stable Isotope-Labeled Internal Standards for RNA Modification Analysis.Genes · 2019Article
- NAIL-MS in E. coli Determines the Source and Fate of Methylation in tRNA.Chembiochem : a European journal of chemical biology · 2018Article
- Mass Spectrometry Analysis of Nucleic Acid Modifications: From Beginning to Future.Mass spectrometry reviewsReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In all domains of life, the nucleobases of tRNA can be methylated. These methylations are introduced either by enzymes or by the reaction of methylating agents with the nucleophilic centers of the nucleobases. Herein, we present a systematic approach to identify the methylation sites within RNA in vitro and in vivo. For discrimination between enzymatic tRNA methylation and tRNA methylation damage in bacteria, we used nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS). With NAIL-MS, we clearly observed the formation of 7-methylguanosine, 3-methyluridine, and 6-methyladenosine during exposure of bacteria to the alkylating agent methyl methanesulfonate (MMS) in vivo. These damage products were not reported to form in tRNA in vivo, as they were masked by the enzymatically formed modified nucleosides in previous studies. In addition, we found formation of the known damage products 1-methyladenosine and 3-methylcytidine in vivo. With a dynamic NAIL-MS setup, we observed tRNA repair by demethylation of these two RNA modifications in vivo. Furthermore, we saw the potential repair of 6-methyladenosine but not 7-methylguanosine in bacterial tRNA.
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