ArticleNature communications2018
Tracing the transitions from pluripotency to germ cell fate with CRISPR screening.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 80 citations in OpenAlex.
- Post-translational modification of proteins in the human testis development pathway.Human reproduction update · 2026Review
- Single cell CRISPR screen identifies antagonism between Nsd1-H3K36me2 and Ezh2-H3K27me3 orchestrates pluripotency transition.Stem cell reports · 2026Article
- Epigenetic blind spots - the role of DNA methylation dynamics in stem cell-based models of embryogenesis.FEBS letters · 2026Article
- A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers.EMBO reports · 2025Article
- Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development.Nature communications · 2025Article
- Generating human primordial germ cell-like cells from pluripotent stem cells: a scoping review ofHuman reproduction open · 2025Review
- The interferon γ pathway enhances pluripotency and X-chromosome reactivation in iPSC reprogramming.Science advances · 2024Article
- An Update on Physiopathological Roles of Akt in the ReprodAKTive Mammalian Ovary.Life (Basel, Switzerland) · 2024Review
- The transcription factor OCT6 promotes the dissolution of the naïve pluripotent state by repressing Nanog and activating a formative state gene regulatory network.Scientific reports · 2024Article
- DNA methylation restricts coordinated germline and neural fates in embryonic stem cell differentiation.Nature structural & molecular biology · 2024Article
- CRISPR loss of function screening to identify genes involved in human primordial germ cell-like cell development.PLoS genetics · 2023Article
- ZMYM2 is essential for methylation of germline genes and active transposons in embryonic development.Nucleic acids research · 2023Article
- Zfp296 knockout enhances chromatin accessibility and induces a unique state of pluripotency in embryonic stem cells.Communications biology · 2023Article
- Epigenetic Reprogramming inCells · 2023Review
- The Novel Role ofInternational journal of molecular sciences · 2023Article
- Esrrb guides naive pluripotent cells through the formative transcriptional programme.Nature cell biology · 2023Article
- Comprehensive chromatin proteomics resolves functional phases of pluripotency and identifies changes in regulatory components.Nucleic acids research · 2023Article
- Mouse primordial germ-cell-like cells lack piRNAs.Developmental cell · 2022Article
- Article
- An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity.Nucleic acids research · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 4 countries.
Funding
Abstract
Early mammalian development entails transit through naive pluripotency towards post-implantation epiblast, which subsequently gives rise to primordial germ cells (PGC), the founding germline population. To investigate these cell fate transitions, we developed a compound-reporter to track cellular identity in a model of PGC specification (PGC-like cells; PGCLC), and coupled it with genome-wide CRISPR screening. We identify key genes both for exit from pluripotency and for acquisition of PGC fate, and characterise a central role for the transcription regulators Nr5a2 and Zfp296 in germline ontogeny. Abrogation of these genes results in widespread activation (Nr5a2
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.