Evidence map›Paper›PMID 30327457›Full record

ArticleNature communications2018

Multi-stage bioengineering of a layered oesophagus with in vitro expanded muscle and epithelial adult progenitors.

Luca Urbani, Carlotta Camilli, Demetra-Ellie Phylactopoulos, Claire Crowley, Dipa Natarajan, Federico Scottoni, Panayiotis Maghsoudlou, Conor J McCann, Alessandro Filippo Pellegata, Anna Urciuolo and 21 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Next-Generation Therapies for Gastrointestinal Repair: Converging Traditional Medicine and Tissue Engineering.JGH open : an open access journal of gastroenterology and hepatology · 2025
    Review
  11. Review
  12. KRT5Stem cell research & therapy · 2025
    Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Tissue Engineering for Gastrointestinal and Genitourinary Tracts.International journal of molecular sciences · 2022
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

31 authors.

Luca UrbaniStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.ORCID http://orcid.org/0000-0002-4988-552X
Carlotta CamilliStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Demetra-Ellie PhylactopoulosStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Claire CrowleyStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Dipa NatarajanStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Federico ScottoniStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.ORCID http://orcid.org/0000-0001-6796-7919
Panayiotis MaghsoudlouStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Conor J McCannStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.ORCID http://orcid.org/0000-0002-9324-5984
Alessandro Filippo PellegataStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.ORCID http://orcid.org/0000-0002-5618-4641
Anna UrciuoloStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Koichi DeguchiStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Sahira KhalafStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Salvatore Ferdinando ArutaStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Maria Cristina SignorelliStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
David KielyStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Edward HannonStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Matteo TrevisanStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.ORCID http://orcid.org/0000-0003-1897-7701
Rui Rachel WongStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Marc Olivier BaradezCell and Gene Therapy Catapult, London, SE1 9RT, UK.
Dale MouldingStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Alex VirasamiDepartment of Histopathology, Great Ormond Street Hospital, University College of London, London, WC1N 3JH, UK.
Asllan GjinovciStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Stavros LoukogeorgakisStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Sara ManteroDepartment of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, 20133, Italy.ORCID http://orcid.org/0000-0002-1264-0465
Nikhil ThaparStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Neil SebireStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.
Simon EatonStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK.ORCID http://orcid.org/0000-0003-0892-9204
Mark LowdellRoyal Free London NHS FT & UCL, London, NW3 2QG, UK.
Giulio CossuDivision of Cell Matrix and Regenerative Medicine, Manchester Academic Health Centre, University of Manchester, Manchester, M13 9PL, UK.
Paola BonfantiStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK. p.bonfanti@ucl.ac.uk.ORCID http://orcid.org/0000-0001-9655-3766
Paolo De CoppiStem Cell and Regenerative Medicine Section, Great Ormond Street Institute of Child Health, University College of London, London, WC1N 1EH, UK. p.decoppi@ucl.ac.uk.

Funding

Medical Research Council MR/P016006/1
6 · The paper itself

Abstract

A tissue engineered oesophagus could overcome limitations associated with oesophageal substitution. Combining decellularized scaffolds with patient-derived cells shows promise for regeneration of tissue defects. In this proof-of-principle study, a two-stage approach for generation of a bio-artificial oesophageal graft addresses some major challenges in organ engineering, namely: (i) development of multi-strata tubular structures, (ii) appropriate re-population/maturation of constructs before transplantation, (iii) cryopreservation of bio-engineered organs and (iv) in vivo pre-vascularization. The graft comprises decellularized rat oesophagus homogeneously re-populated with mesoangioblasts and fibroblasts for the muscle layer. The oesophageal muscle reaches organised maturation after dynamic culture in a bioreactor and functional integration with neural crest stem cells. Grafts are pre-vascularised in vivo in the omentum prior to mucosa reconstitution with expanded epithelial progenitors. Overall, our optimised two-stage approach produces a fully re-populated, structurally organized and pre-vascularized oesophageal substitute, which could become an alternative to current oesophageal substitutes.

Indexed as

Tissue ScaffoldsAnimalsCell Culture TechniquesCell DifferentiationChildChild, PreschoolCryopreservationEpithelial CellsEsophagusExtracellular MatrixHumansInfantInfant, NewbornMaleMice, Inbred C57BLMice, Transgenic

Identifiers

PMID30327457
PMCPMC6191423

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.