ArticleBlood advances2018
Absolute proteome quantification of highly purified populations of circulating reticulocytes and mature erythrocytes.
Article in Blood advances, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed, 103 citations in OpenAlex.
- Article
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- Post-translational Modifications in Proteins: Prediction Methods, Biological Functions, and Diseases.MedComm · 2026Review
- Longitudinal metabolomics study of phosphate-adenine-guanosine-glucose-saline-mannitol stored red blood cells.Transfusion · 2026Article
- Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics.Scientific reports · 2026Article
- Piezo1 dictates KProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Comprehensive Proteomics Profiling Reveals Biomarkers of Oxidative Stress and Membrane Integrity in Red Blood Cells in Acute Altitude Sickness.ACS omega · 2025Article
- Flavin-deficient erythrocytes offer protection against malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Comprehensive Phenotypic and Proteomic Analyses of Human Reticulocyte Maturation.Blood red cells & iron · 2025Article
- Advances on the genetic basis of red cell membrane disorders.Current opinion in hematology · 2025Review
- Role of channels in the ObioRxiv : the preprint server for biology · 2025Article
- In vitro studies of human erythropoiesis using a 3D silk-based bone marrow model that generates erythroblastic islands.Blood advances · 2025Article
- Proteostasis and metabolic dysfunction characterize a subset of storage-induced senescent erythrocytes targeted for posttransfusion clearance.The Journal of clinical investigation · 2025Article
- Human serum albumin or polyvinyl alcohol can only partially replace human plasma during in vitro red cell production from PBMC.Scientific reports · 2025Article
- A replication study of novel fetal hemoglobin-associated genetic variants in sickle cell disease-only cohorts.Human molecular genetics · 2025Article
- Emerging concepts in the molecular cell biology and functions of mammalian erythrocytes.The Journal of biological chemistry · 2025Review
- RBC-GEM: A genome-scale metabolic model for systems biology of the human red blood cell.PLoS computational biology · 2025Article
- Insights from lipidomics into the terminal maturation of circulating human reticulocytes.Cell death discovery · 2025Article
- Modulation of Antioxidant Enzyme Expression of In Vitro Culture-Derived Reticulocytes.Antioxidants (Basel, Switzerland) · 2024Article
6 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 6 institutions in 2 countries.
Funding
Abstract
Reticulocytes produced in the bone marrow undergo maturation in the bloodstream to give rise to erythrocytes. Although the proteome of circulating red cells has been the subject of several reports, the cellular populations used for these studies were never completely devoid of reticulocytes. In our current study, we used highly purified erythrocyte and reticulocyte populations to quantify the absolute expression levels of the proteins in each cell population. Erythrocytes and reticulocytes were purified in a multistep process involving cellulose chromatography, Percoll gradient centrifugation, and fluorescence cell sorting after thiazole orange labeling. Proteins were analyzed by mass spectrometry from whole cells and erythrocyte plasma membrane (ghosts), leading to the identification and quantification of 2077 proteins, including 654 that were reticulocyte-specific. Absolute quantifications of these proteins were made using the mean corpuscular hemoglobin content of the cells as a standard. For each protein, we calculated the percentage loss during the terminal stages of reticulocyte maturation and the percentage of association with the plasma membrane. In addition, we used modified adenosine triphosphate and adenosine diphosphate molecules that enable the transfer of a biotin molecule to the catalytic sites of kinases to isolate active kinases in the erythrocytes and determined the absolute expression of 75 protein kinases and the modification of their expression during reticulocyte maturation. Our findings represent the first absolute quantification of proteins that are specifically expressed in normal erythrocytes with no detectable contamination by reticulocytes. Our findings thus represent a reference database for the future proteomic analysis of pathological erythrocytes.
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