Evidence map›Paper›PMID 30324591›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

Imaging Cytonemes in Drosophila Embryos.

Lijuan Du, Sougata Roy

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Lijuan DuDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA.
Sougata RoyDepartment of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD, USA. sougata@umd.edu.
University of Maryland, College Park · US

Funding

Mechanisms of Cell Communication in Animal DevelopmentR35GM124878 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI ROY, SOUGATA · 2017 to 2021
$2.1M
Drosophila tracheal cytonemes in long range FGF signalingR00HL114867 · NHLBI · UNIV OF MARYLAND, COLLEGE PARK · PI ROY, SOUGATA · 2014 to 2016
$696k
NHLBI NIH HHS R00 HL114867NIGMS NIH HHS R35 GM124878
6 · The paper itself

Abstract

Conserved morphogenetic signaling proteins disperse across tissues to generate signal and signaling gradients, which in turn are considered to assign positional coordinates to the recipient cells. Recent imaging studies in Drosophila model have provided evidence for a "direct-delivery" mechanism of signal dispersion that is mediated by specialized actin-rich signaling filopodia, named cytonemes. Cytonemes establish contact between the signal-producing and target cells to directly exchange and transport the morphogenetic proteins. Although an increasing amount of evidence supports the critical role of these specialized signaling structures, imaging these highly dynamic 200 nm-thin structures in the complex three-dimensional contour of living tissues is challenging. Here, we describe the imaging methods that we optimized for studying cytonemes in Drosophila embryos.

Indexed as

AnimalsCell CommunicationDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianFemaleImage Processing, Computer-AssistedMaleMicroscopy, ConfocalMorphogenesisPseudopodiaSignal TransductionDrosophila ProteinsBreathlessCell–cell signalingCytonemesDrosophilaEmbryoEngrailedFilopodiaMorphogenTrachea

Identifiers

PMID30324591
PMCPMC6534123
OpenAlexW2897378328

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.