ArticleJournal of bone and mineral metabolism2019
Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators.
Article in Journal of bone and mineral metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Modulation of TNAP activity and apatite formation in biomimetic matrix vesicles studied byBiochimica et biophysica acta. Biomembranes · 2025Article
- Effect of sugarcane cystatin CaneCPI-5 on osteogenic differentiation of human dental pulp cells.Brazilian dental journal · 2025Article
- Annexin A5 stabilizes matrix vesicle-biomimetic lipid membranes: unravelling a new role of annexins in calcification.European biophysics journal : EBJ · 2023Article
- NPP1 and TNAP hydrolyze ATP synergistically during biomineralization.Purinergic signalling · 2023Article
- Shedding Light on the Role of Na,K-ATPase as a Phosphatase during Matrix-Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- Mineralization Profile of Annexin A6-Harbouring Proteoliposomes: Shedding Light on the Role of Annexin A6 on Matrix Vesicle-Mediated Mineralization.International journal of molecular sciences · 2022Article
- The functional role of soluble proteins acquired by extracellular vesicles.Journal of extracellular biology · 2022Review
- Langmuir monolayers and proteoliposomes as models of matrix vesicles involved in biomineralization.Biophysical reviews · 2021Article
- In Search of a Role for Extracellular Purine Enzymes in Bone Function.Biomolecules · 2021Review
- Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization.PLoS computational biology · 2021Article
- Lipid composition modulates ATP hydrolysis and calcium phosphate mineral propagation by TNAP-harboring proteoliposomes.Archives of biochemistry and biophysics · 2020Article
- Cholesterol Regulates the Incorporation and Catalytic Activity of Tissue-Nonspecific Alkaline Phosphatase in DPPC Monolayers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.Archives of biochemistry and biophysics · 2019Article
- Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?Archives of biochemistry and biophysics · 2019Article
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10 authors.
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Abstract
Tissue-nonspecific alkaline phosphatase (TNAP), a glycosylphosphatidylinositol-anchored ectoenzyme present on the membrane of matrix vesicles (MVs), hydrolyzes the mineralization inhibitor inorganic pyrophosphate as well as ATP to generate the inorganic phosphate needed for apatite formation. Herein, we used proteoliposomes harboring TNAP as MV biomimetics with or without nucleators of mineral formation (amorphous calcium phosphate and complexes with phosphatidylserine) to assess the role of the MVs' membrane lipid composition on TNAP activity by means of turbidity assay and FTIR analysis. We found that TNAP-proteoliposomes have the ability to induce mineralization even in the absence of mineral nucleators. We also found that the addition of cholesterol or sphingomyelin to TNAP-proteoliposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine reduced the ability of TNAP to induce biomineralization. Our results suggest that the lipid microenvironment is essential for the induction and propagation of minerals mediated by TNAP.
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