ArticleStem cell reviews and reports2019
Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation.
Article in Stem cell reviews and reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 98 citations in OpenAlex.
- Chemically Defined Medium Enables GDNF-Driven Early Neuronal-like Phenotype of Human Dental Pulp Stem Cells.Cells · 2026Article
- Optimizing neurogenic differentiation of dental stem cells: a critical review of induction strategies and functional validation.Frontiers in dental medicine · 2026Review
- Mesenchymal Stromal Cells from Dental Tissues Demonstrate Neuronal Potential Pre- and Post-Induction.Cells, tissues, organs · 2026Article
- Molecular Mechanisms and Clinical Applications of Neural Regeneration Through Dental Pulp Stem Cells.Stem cells international · 2026Review
- Neuronal Differentiation and Exosome Profiling of Dental Pulp Stem Cells: Unveiling Their Potential for Nerve Repair.International journal of molecular sciences · 2025Article
- Scaffold-Free Strategies in Dental Pulp/Dentine Tissue Engineering: Current Status and Implications for Regenerative Biological Processes.Bioengineering (Basel, Switzerland) · 2025Review
- Functional differentiation of human dental pulp stem cells into neuron-like cells exhibiting electrophysiological activity.Stem cell research & therapy · 2025Article
- Mesenchymal stem cells and their extracellular vesicle therapy for neurological disorders: traumatic brain injury and beyond.Frontiers in neurology · 2025Review
- The role of neurogenic inflammation in pulp repair and the techniques used for its assessment (narrative review).Frontiers in dental medicine · 2025Review
- The temporospatial relationship between mouse dental pulp stem cells and tooth innervation.Journal of dental sciences · 2024Article
- The nuclei of human adult stem cells can move within the cell and generate cellular protrusions to contact other cells.Stem cell research & therapy · 2024Article
- The potential therapeutic roles of dental pulp stem cells in spinal cord injury.Frontiers in molecular biosciences · 2024Review
- Therapeutic Potential of Oral-Derived Mesenchymal Stem Cells in Retinal Repair.Stem cell reviews and reports · 2023Review
- Review
- Clinical trials using dental stem cells: 2022 update.World journal of stem cells · 2023Review
- Oral cavity-derived stem cells and preclinical models of jaw-bone defects for bone tissue engineering.Stem cell research & therapy · 2023Review
- The Wisdom in Teeth: Neuronal Differentiation of Dental Pulp Cells.Cellular reprogramming · 2023Review
- Local transplantation of GMSC-derived exosomes to promote vascularized diabetic wound healing by regulating the Wnt/β-catenin pathways.Nanoscale advances · 2023Article
- Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction.Journal of applied oral science : revista FOB · 2023Article
- Function and mechanism of mesenchymal stem cells in the healing of diabetic foot wounds.Frontiers in endocrinology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
The potential of human mesenchymal stromal/stem cells (MSCs) including oral stem cells (OSCs) as a cell source to derive functional neurons has been inconclusive. Here we tested a number of human OSCs for their neurogenic potential compared to non-OSCs and employed various neurogenic induction methods. OSCs including dental pulp stem cells (DPSCs), gingiva-derived mesenchymal stem cells (GMSCs), stem cells from apical papilla and non-OSCs including bone marrow MSCs (BMMSCs), foreskin fibroblasts and dermal fibroblasts using non-neurosphere-mediated or neurosphere-mediated methods to guide them toward neuronal lineages. Cells were subjected to RT-qPCR, immunocytofluorescence to detect the expression of neurogenic genes or electrophysiological analysis at final stage of maturation. We found that induced DPSCs and GMSCs overall appeared to be more neurogenic compared to other cells either morphologically or levels of neurogenic gene expression. Nonetheless, of all the neural induction methods employed, only one neurosphere-mediated method yielded electrophysiological properties of functional neurons. Under this method, cells expressed increased neural stem cell markers, nestin and SOX1, in the first phase of differentiation. Neuronal-like cells expressed βIII-tubulin, CNPase, GFAP, MAP-2, NFM, pan-Nav, GAD67, Nav1.6, NF1, NSE, PSD95, and synapsin after the second phase of differentiation to maturity. Electrophysiological experiments revealed that 8.3% of DPSC-derived neuronal cells and 21.2% of GMSC-derived neuronal cells displayed action potential, although no spontaneous excitatory/inhibitory postsynaptic action potential was observed. We conclude that DPSCs and GMSCs have the potential to become neuronal cells in vitro, therefore, these cells may be used as a source for neural regeneration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.