Construction of a Novel DNA Vaccine Candidate encoding LmSTI1-PpSP42 Fusion Protein from

Touraj Miandoabi, Fariborz Bahrami, Vahideh Moein Vaziri, Soheila Ajdary

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Article in Reports of biochemistry & molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

–field-weighted citation impact, top 95% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

Bulky pcDNA plasmid encoding Leishmania- and Sandfly-derived antigens resulted in transient Th1 response post Leishmania major infection in BALB/c mice.Scientific reports · 2026
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Touraj MiandoabiDepartment of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Fariborz BahramiPasteur Institute of Iran, Department of Immunology, 69 Pasteur Ave., Tehran, Iran.

Vahideh Moein VaziriDepartment of Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Soheila AjdaryPasteur Institute of Iran, Department of Immunology, 69 Pasteur Ave., Tehran, Iran.

Shahid Beheshti University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Methods: The nucleotide sequences encoding the LmSTI1 protein and a fragment encoding 79% of PpSP42 were amplified using Results: Sequences encoding LmSTI1 and partial PpSP42 were cloned into pcDNA3.1(+). Production of the recombinant LmSTI1Pp42 fusion protein was confirmed by Western blotting. Conclusion: An LmSTI1Pp42 fusion protein was expressed HEK-293T cells. This construct may be an effective DNA vaccine against CL.

Indexed as

CloningDNA vaccineLeishmania majorLmSTI1PpSP42

Identifiers

PMID30324120

PMCPMC6175588

OpenAlexW2796537825

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.