ArticleFrontiers in neuroanatomy2018
Fast Homogeneous
Article in Frontiers in neuroanatomy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Active repression of muscle fate preserves neural lineage identity during cerebellum development.bioRxiv : the preprint server for biology · 2026Article
- NucleoNet and DropNet: Generalist deep learning models for instance segmentation of nuclei and lipid droplets from electron microscopy images.bioRxiv : the preprint server for biology · 2026Article
- Optimisation of freeze substitution protocols for the examination of malaria parasite structure by volumetric electron microscopy.Journal of microscopy · 2026Article
- Correlative light and electron microscopy for human brain and other biological models.Nature protocols · 2025Review
- Transfer learning improves performance in volumetric electron microscopy organelle segmentation across tissues.Bioinformatics advances · 2025Article
- Structural brain preservation: a potential bridge to future medical technologies.Frontiers in medical technology · 2024Article
- Rehydration of Freeze Substituted Brain Tissue for Pre-embedding Immunoelectron Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Minimal resin embedding of SBF-SEM samples reduces charging and facilitates finding a surface-linked region of interest.Frontiers in zoology · 2023Article
- Femtosecond laser preparation of resin embedded samples for correlative microscopy workflows in life sciences.Applied physics letters · 2023Article
- Article
- How innovations in methodology offer new prospects for volume electron microscopy.Journal of microscopy · 2022Review
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- Identifying long-range synaptic inputs using genetically encoded labels and volume electron microscopy.Scientific reports · 2022Article
- A versatile enhanced freeze-substitution protocol for volume electron microscopy.Frontiers in cell and developmental biology · 2022Article
- Reconstructing neural circuits using multiresolution correlated light and electron microscopy.Frontiers in neural circuits · 2022Article
- Sample Preparation and Warping Accuracy for Correlative Multimodal Imaging in the Mouse Olfactory Bulb Using 2-Photon, Synchrotron X-Ray and Volume Electron Microscopy.Frontiers in cell and developmental biology · 2022Article
- Field-Emission Scanning Electron Microscope as a Tool for Large-Area and Large-Volume Ultrastructural Studies.Animals : an open access journal from MDPI · 2021Review
- Adaptive cell invasion maintains lateral line organ homeostasis in response to environmental changes.Developmental cell · 2021Article
- Upscaling X-ray nanoimaging to macroscopic specimens.Journal of applied crystallography · 2021Review
- Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fixation and staining of large tissue samples are critical for the acquisition of volumetric electron microscopic image datasets and the subsequent reconstruction of neuronal circuits. Efficient protocols exist for the staining of small samples, but uniform contrast is often difficult to achieve when the sample diameter exceeds a few hundred micrometers. Recently, a protocol (BROPA, brain-wide reduced-osmium staining with pyrogallol-mediated amplification) was developed that achieves homogeneous staining of the entire mouse brain but requires very long sample preparation times. By exploring modifications of this protocol we developed a substantially faster procedure, fBROPA, that allows for reliable high-quality staining of tissue blocks on the millimeter scale. Modifications of the original BROPA protocol include drastically reduced incubation times and a lead aspartate incubation to increase sample conductivity. Using this procedure, whole brains from adult zebrafish were stained within 4 days. Homogenous high-contrast staining was achieved throughout the brain. High-quality image stacks with voxel sizes of 10 × 10 × 25 nm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.