Evidence map›Paper›PMID 30323594›Full record

ArticleInternational journal of nanomedicine2018

Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles.

Megan A Yamoah, Maryam Moshref, Janhavi Sharma, Wei Chun Chen, Hannah A Ledford, Jeong Han Lee, Karen S Chavez, Wenying Wang, Javier E López, Deborah K Lieu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of nanomedicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Megan A YamoahDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Maryam MoshrefDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Janhavi SharmaDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Wei Chun ChenDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Hannah A LedfordDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Jeong Han LeeDepartment of Physiology and Cell Biology, University of Nevada, Reno, NV, USA.
Karen S ChavezDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Wenying WangDepartment of Physiology and Cell Biology, University of Nevada, Reno, NV, USA.
Javier E LópezDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Deborah K LieuDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Padmini SirishDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
Xiao-Dong ZhangDepartment of Internal Medicine, University of California, Davis, CA, USA, xdzhang@ucdavis.edu, psirish@ucdavis.edu.
University of California, Davis · USUniversity of Nevada, Reno · USVA Northern California Health Care System · US

Funding

UC Davis Clinical and Translational Science CenterUL1TR000002 · NCATS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BERGLUND, LARS F · 2012 to 2015
$12.2M
Functional Regulation of Cardiac Chloride HomeostasisR56HL138392 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ZHANG, XIAO-DONG · 2018 to 2018
$393k
RNF207: A Potential Modifier of HERG in Long QT SyndromeF31HL136120 · NHLBI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEDFORD, HANNAH AIZAD · 2017 to 2018
$54k
NCATS NIH HHS UL1 TR000002NHLBI NIH HHS F31 HL136120NHLBI NIH HHS R56 HL138392
6 · The paper itself

Abstract

purposeThe delivery of transgenes into human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) represents an important tool in cardiac regeneration with potential for clinical applications. Gene transfection is more difficult, however, for hiPSCs and hiPSC-CMs than for somatic cells. Despite improvements in transfection and transduction, the efficiency, cytotoxicity, safety, and cost of these methods remain unsatisfactory. The objective of this study is to examine gene transfection in hiPSCs and hiPSC-CMs using magnetic nanoparticles (NPs).

methodsMagnetic NPs are unique transfection reagents that form complexes with nucleic acids by ionic interaction. The particles, loaded with nucleic acids, can be guided by a magnetic field to allow their concentration onto the surface of the cell membrane. Subsequent uptake of the loaded particles by the cells allows for high efficiency transfection of the cells with nucleic acids. We developed a new method using magnetic NPs to transfect hiPSCs and hiPSC-CMs. HiPSCs and hiPSC-CMs were cultured and analyzed using confocal microscopy, flow cytometry, and patch clamp recordings to quantify the transfection efficiency and cellular function.

resultsWe compared the transfection efficiency of hiPSCs with that of human embryonic kidney (HEK 293) cells. We observed that the average efficiency in hiPSCs was 43%±2% compared to 62%±4% in HEK 293 cells. Further analysis of the transfected hiPSCs showed that the differentiation of hiPSCs to hiPSC-CMs was not altered by NPs. Finally, robust transfection of hiPSC-CMs with an efficiency of 18%±2% was obtained.

conclusionThe difficult-to-transfect hiPSCs and hiPSC-CMs were efficiently transfected using magnetic NPs. Our study offers a novel approach for transfection of hiPSCs and hiPSC-CMs without the need for viral vector generation.

Indexed as

Cell DifferentiationHEK293 CellsHumansInduced Pluripotent Stem CellsLipidsMagnetite NanoparticlesMyocytes, CardiacTransfectionLipidsLipofectamineMagnetite Nanoparticlesefficiencyhuman induced pluripotent stem cell-derived cardiomyocytespluripotencytherapy

Identifiers

PMID30323594
PMCPMC6179720
OpenAlexW2894731159

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.