ArticleScientific reports2018
Fine-tuning carbapenem resistance by reducing porin permeability of bacteria activated in the selection process of conjugation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Resolving competing evolutionary histories in joint ancestral state reconstruction.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The EnvZ/OmpR two-component regulatory system regulates biofilm formation inFrontiers in microbiology · 2026Article
- Uncertainty in joint Ancestral State Reconstruction: Improving accuracy and biological interpretability of ancestral state prediction.bioRxiv : the preprint server for biology · 2025Article
- Chromosomal resistance mutations facilitate acquisition of multidrug-resistant plasmids inMicrobiology (Reading, England) · 2025Article
- ESBL-ProducingMicroorganisms · 2025Article
- Determining the potential targets of silybin by molecular docking and its antibacterial functions on efflux pumps and porins in uropathogenic E. coli.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Next-Gen Nano Biosensor Technologies to Monitor Carbapenem Resistance for Personalized Medicine.Indian journal of microbiology · 2025Article
- Porin deficiency or plasmid copy number increase mediated carbapenem-resistantEmerging microbes & infections · 2024Article
- Carbapenem-ResistantMedicina (Kaunas, Lithuania) · 2024Review
- Multidrug-Resistant and Extensively Drug-ResistantMicroorganisms · 2023Article
- Characterization of Genomic Diversity among Carbapenem-ResistantMicroorganisms · 2022Article
- Extensively Drug-Resistant Klebsiella pneumoniae Counteracts Fitness and Virulence Costs That Accompanied Ceftazidime-Avibactam Resistance Acquisition.Microbiology spectrum · 2022Article
- Genomic Analysis of Antimicrobial Resistance and Resistance Plasmids inAntibiotics (Basel, Switzerland) · 2021Article
- Distribution of β-Lactamase Genes and Genetic Context ofInfection and drug resistance · 2021Article
- Emerging Transcriptional and Genomic Mechanisms Mediating Carbapenem and Polymyxin Resistance inmSystems · 2020Review
- Antibiotic resistance and persistence-Implications for human health and treatment perspectives.EMBO reports · 2020Review
- Transcriptome-based design of antisense inhibitors potentiates carbapenem efficacy in CREProceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Antibiotic resistance is an emerging public health issue. Plasmids are one of the popular carriers to disseminate resistance genes among pathogens. However, the response of plasmid-carrying bacteria to antibiotic treatment and how these bacteria evolve to increase their resistance remain elusive. In this study, we conjugated plasmid pNDM-HK to E. coli J53 recipient cells and selected survivors using different concentrations of the broad spectrum antibiotic meropenem. After selection, transconjugants conferred varying minimum inhibitory concentrations with respect to carbapenems. We sequenced and compared the transcriptomes of transconjugants that exhibited distinct carbapenem susceptibilities, and found that the loss of outer membrane proteins led to antibiotic resistance. Moreover, we identified a novel mutation, G63S, in transcription factor OmpR which moderates the expression of outer membrane proteins. The loss of porins was due to incapability of phosphorylation, which is essential for porin transcription and carbapenem resistance. We also characterized other genes that are regulated by ompR in this mutant, which contributed to bacterial antibiotic resistance. Overall, our studies suggest antibiotic pressure after conjugation might be an alternative pathway to promote antimicrobial resistance.
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