ArticleJournal of lipid research2018
Quantitative determination of esterified eicosanoids and related oxygenated metabolites after base hydrolysis.
Article in Journal of lipid research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Influence of Dietary n-3 Long Chain Polyunsaturated Fatty Acid Intake on Oxylipins in Erythrocytes of Women with Rheumatoid Arthritis.Molecules (Basel, Switzerland) · 2023Trial
- Flavonoids as Modulators of Lipid Mediator Signaling: A Narrative Review on Mechanisms, Current Evidence, and Gaps in the Literature.Molecular nutrition & food research · 2026Review
- Lean adipocyte oxylipin signaling restrains breast cancer through ferroptosis.Science (New York, N.Y.) · 2026Article
- Total oxylipin concentrations in NIST SRM 1950 plasma compared to fresh and commercially obtained human plasma.Journal of lipid research · 2026Article
- An updated overview of lipid-regulated immunobiology in macrophages.Acta biochimica et biophysica Sinica · 2026Review
- A Method for Analysis of Oxidized Phospholipids from Biological Samples Using Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- From MS/MS library implementation to molecular networks: Exploring oxylipin diversity with NEO-MSMS.Scientific data · 2024Article
- Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.Science immunology · 2021Article
- Triacylglycerols are preferentially oxidized over free fatty acids in heated soybean oil.NPJ science of food · 2021Article
- Linoleic acid-derived 13-hydroxyoctadecadienoic acid is absorbed and incorporated into rat tissues.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Article
- Harmonized procedures lead to comparable quantification of total oxylipins across laboratories.Journal of lipid research · 2020Article
- Non-targeted and targeted analysis of oxylipins in combination with charge-switch derivatization by ion mobility high-resolution mass spectrometry.Analytical and bioanalytical chemistry · 2020Article
- Molecular changes in transcription and metabolic pathways underlying muscle atrophy in the CuZnSOD null mouse model of sarcopenia.GeroScience · 2020Article
- Plasma eicosanoids as noninvasive biomarkers of liver fibrosis in patients with nonalcoholic steatohepatitis.Therapeutic advances in gastroenterology · 2020Article
- "Redox lipidomics technology: Looking for a needle in a haystack".Chemistry and physics of lipids · 2019Review
- Diet Supplementation in ω3 Polyunsaturated Fatty Acid Favors an Anti-Inflammatory Basal Environment in Mouse Adipose Tissue.Nutrients · 2019Article
- Extracellular Vesicles From Auditory Cells as Nanocarriers for Anti-inflammatory Drugs and Pro-resolving Mediators.Frontiers in cellular neuroscience · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Eicosanoids and related metabolites (oxylipins) possess potent signaling properties, elicit numerous important physiologic responses, and serve as biomarkers of disease. In addition to their presence in free form, a considerable portion of these bioactive lipids is esterified to complex lipids in cell membranes and plasma lipoproteins. We developed a rapid and sensitive method for the analysis of esterified oxylipins using alkaline hydrolysis to release them followed by ultra-performance LC coupled with mass spectrometric analysis. Detailed evaluation of the data revealed that several oxylipins are susceptible to alkaline-induced degradation. Nevertheless, of the 136 metabolites we examined, 56 were reproducibly recovered after alkaline hydrolysis. We classified those metabolites that were resistant to alkaline-induced degradation and applied this methodology to quantify metabolite levels in a macrophage cell model and in plasma of healthy subjects. After alkaline hydrolysis of lipids, 34 metabolites could be detected and quantified in resting and activated macrophages, and 38 metabolites were recovered from human plasma at levels that were substantially greater than in free form. By carefully selecting internal standards and taking the observed experimental limitations into account, we established a robust method that can be reliably employed for the measurement of esterified oxylipins in biological samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.