ArticleInternational journal of molecular sciences2018
PEG-Plasma Hydrogels Increase Epithelialization Using a Human Ex Vivo Skin Model.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Human Wound Healing Ex Vivo Model with Focus on Molecular Markers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Blood Plasma, Fibrinogen or Fibrin Biomaterial for the Manufacturing of Skin Tissue-Engineered Products and Other Dermatological Treatments: A Systematic Review.Journal of functional biomaterials · 2025Review
- Biodegradable Silk Fibroin Matrices for Wound Closure in a Human 3D Ex Vivo Approach.Materials (Basel, Switzerland) · 2024Article
- A frog peptide provides new strategies for the intervention against skin wound healing.Cellular & molecular biology letters · 2023Article
- Article
- Application of an instructive hydrogel accelerates re-epithelialization of xenografted human skin wounds.Scientific reports · 2022Article
- Preparation and Characterization of Plasma-Derived Fibrin Hydrogels Modified by Alginate di-Aldehyde.International journal of molecular sciences · 2022Article
- Safety and efficacy of acellular human amniotic fluid and membrane in the treatment of non-healing wounds in a patient with chronic venous insufficiency.SAGE open medical case reports · 2022Article
- Elastin-Plasma Hybrid Hydrogels for Skin Tissue Engineering.Polymers · 2021Article
- Effect of Fibrin Concentration on the In Vitro Production of Dermo-Epidermal Equivalents.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In vitro cell culture methods are used extensively to study cellular migration, proliferation, and differentiation, which play major roles in wound healing but the results often do not translate to the in vivo environment. One alternative would be to establish an ex vivo model utilizing human discarded skin to evaluate therapies in a more natural setting. The purpose of this study was to institute such a model by creating 'wounds' in the center of a piece of discarded skin and treating them with three different biomaterials: collagen, polyethylene glycol (PEG)-fibrin, or PEG-platelet free plasma (PFP). Explants were cultured for 14 days with supernatant and microscopy images collected every 3 days to assess cytotoxicity and epithelialization. After 14 days, the explants were fixed, sectioned, and stained for cytokeratin-10 (CK-10), alpha-smooth muscle actin (α-SMA), and wheat germ (WG). Compared to controls, similar levels of cytotoxicity were detected for 12 days which decreased slightly at day 14. The PEG-PFP hydrogel-treated wounds epithelialized faster than other treatments at days 6 to 14. A 6-8 cell layer thick CK-10+ stratified epidermis had developed over the PEG-PFP hydrogel and cells co-stained by WG and α-SMA were observed within the hydrogel. An ex vivo model was established that can be used practically to screen different therapies exploring wound healing.
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