ArticleThe FEBS journal2018
The auxin-inducible degradation system enables conditional PERIOD protein depletion in the nervous system of Drosophila melanogaster.
Article in The FEBS journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Chemical biology approaches to study and target circadian clocks and their components.FEBS letters · 2026Review
- Protein Depletion in Caenorhabditis elegans Using the Auxin-Inducible Degradation System.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Comprehensive insights into the impact of bacterial indole-3-acetic acid on sensory preferences in Drosophila melanogaster.Scientific reports · 2024Article
- The emergence of circadian timekeeping in the intestine.Nature communications · 2024Article
- Article
- Seasonal cues act through the circadian clock and pigment-dispersing factor to control EYES ABSENT and downstream physiological changes.Current biology : CB · 2023Article
- Phosphorylation Promotes the Accumulation of PERIOD Protein Foci.Research (Washington, D.C.) · 2023Article
- A spontaneousmicroPublication biology · 2023Article
- Article
- Controlling CRISPR with small molecule regulation for somatic cell genome editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Review
- Article
- High-Salt Diet Impairs the Neurons Plasticity and the Neurotransmitters-Related Biological Processes.Nutrients · 2021Article
- Clock proteins regulate spatiotemporal organization of clock genes to control circadian rhythms.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Efficient generation of a single-copymicroPublication biology · 2021Article
- Rapid Degradation ofG3 (Bethesda, Md.) · 2020Article
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3 authors.
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Abstract
Tools that allow inducible and reversible depletion of target proteins are critical for biological studies. The plant-derived auxin-inducible degradation system (AID) enables the degradation of target proteins tagged with the AID motif. This system has been recently employed in mammalian cells as well as in Caenorhabditis elegans and Drosophila. To test the utility of the AID approach in the nervous system, we used circadian locomotor rhythms as a model and applied the AID method to temporally and spatially degrade PERIOD (PER), a critical pacemaker protein in Drosophila. We found that the period locus can be efficiently tagged with the AID motif by CRISPR/Cas9-based genome editing without disrupting PER function. Moreover, we demonstrated that the AID system could be used to induce rapid and efficient protein degradation in the nervous system as shown by effects on circadian and sleep behaviors. Furthermore, the protein degradation by AID was rapidly reversible after auxin removal. Together, our results show that the AID system provides a powerful tool for behavior studies in Drosophila.
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