ArticleToxicological sciences : an official journal of the Society of Toxicology2019
Application of Benchmark Concentration (BMC) Analysis on Zebrafish Data: A New Perspective for Quantifying Toxicity in Alternative Animal Models.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Zebrafish behavioral assessment as a first-tier whole-organism NAM for developmental neurotoxicity: A multi-laboratory evaluation.Neurotoxicology · 2026Article
- Prioritizing chemicals for developmental neurotoxicity by integrating data from a new approach methods (NAMs) battery covering key cellular events in neurodevelopment.Neurotoxicology · 2026Article
- Power calculations for larval zebrafish in light-dark transition test for developmental neurotoxicity.Neurotoxicology · 2026Article
- Rapid behavioral screening in the planarianFrontiers in toxicology · 2026Article
- Red Midge Larvae Are an Invertebrate Alternative Diet to Beef Liver for Planarian Husbandry.Biomolecules · 2025Article
- Statistical analysis of multi-endpoint phenotypic screening increases sensitivity of planarian neurotoxicity testing.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- A comparative study of biostatistical pipelines for benchmark concentration modeling ofComputational toxicology (Amsterdam, Netherlands) · 2025Article
- Methylene blue at recommended concentrations alters metabolism in early zebrafish development.Communications biology · 2025Article
- A data analysis approach for locomotor behavioral responses in developmentally exposed zebrafish.NAM journal · 2025Article
- Article
- Investigation of Peroxisome Proliferator-Activated Receptor Genes as Requirements for Visual Startle Response Hyperactivity in Larval Zebrafish Exposed to Structurally Similar Per- and Polyfluoroalkyl Substances (PFAS).Environmental health perspectives · 2024Article
- How neurobehavior and brain development in alternative whole-organism models can contribute to prediction of developmental neurotoxicity.Neurotoxicology · 2024Review
- Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish.Scientific reports · 2024Article
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- Article
- Exploring the Influence of Experimental Design on Toxicity Outcomes in Zebrafish Embryo Tests.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Article
- Differences in neurotoxic outcomes of organophosphorus pesticides revealedFrontiers in toxicology · 2022Article
- Accounting for Artifacts in High-Throughput Toxicity Assays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Use of Zebrafish in Drug Discovery Toxicology.Chemical research in toxicology · 2020Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Over the past decade, the zebrafish is increasingly being used as a model to screen for chemical-mediated toxicities including developmental toxicity (DT) and neurotoxicity (NT). One of the major challenges is lack of harmonization in data analysis approaches, thereby posing difficulty in comparing findings across laboratories. To address this, we sought to establish a unified data analysis strategy for both DT and NT data, by adopting the benchmark concentration (BMC) analysis. There are two critical aspects in the BMC analysis: having a toxicity endpoint amenable for BMC and selecting a proper benchmark response (BMR) for the endpoint. For the former, in addition to the typical endpoints in NT assay (eg, hyper/hypo- response quantified by distance moved), we also used endpoints that assess the differences in movement patterns between chemical-treated embryos and control embryos. For the latter, we standardized the selection of BMR, which is analogous to minimum activity threshold, based on intrinsic response variations in the endpoint. When comparing our BMC results with a traditionally used LOAEL method (lowest-observed-adverse-effect level), we found high active compound concordance (100% for DT vs 74% for NT); generally, the BMC was more sensitive than LOAEL (no. of BMC more sensitive/no. of concordant active compounds, 43/50 for DT vs 16/26 for NT). Using the BMC with standardized toxicity endpoints and an appropriate BMR, we may now have a unified data-analysis approach to comparing results across different zebrafish datasets, for a better understanding of strengths and challenges when using the zebrafish as a screening tool.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.