ArticleBiomedical optics express2018
Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model.
Article in Biomedical optics express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- A Beginner's Guide to Cardiac Optical Mapping.Cardiovascular engineering and technology · 2026Review
- Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy.Diagnostics (Basel, Switzerland) · 2026Review
- Multiplexed Fiber-Optic Fluorescence for Functional Monitoring of Perfused Hearts.Analytical chemistry · 2025Article
- Multimodal Optical Imaging Combined with Radiomic Analysis for Fibrotic Cardiac Tissue Investigation.Analytical chemistry · 2025Article
- Fluorescence lifetime imaging microscopy of endogenous fluorophores in health and disease.Journal of muscle research and cell motility · 2025Review
- Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens.Scientific reports · 2024Article
- Dual excitation spectral autofluorescence lifetime and reflectance imaging for fast macroscopic characterization of tissues.Biomedical optics express · 2024Article
- The potential roles of stress-induced phosphoprotein 1 and connexin 43 in rats with reperfusion arrhythmia.Immunity, inflammation and disease · 2023Article
- Graphene Biointerface for Cardiac Arrhythmia Diagnosis and Treatment.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Label-free and noninvasive method for assessing the metabolic status in type 2 diabetic rats with myocardium diastolic dysfunction.Biomedical optics express · 2021Article
- Real-time multispectral fluorescence lifetime imaging using Single Photon Avalanche Diode arrays.Scientific reports · 2020Article
- Discriminating different grades of cervical intraepithelial neoplasia based on label-free phasor fluorescence lifetime imaging microscopy.Biomedical optics express · 2020Article
- Fast fit-free analysis of fluorescence lifetime imaging via deep learning.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- In vivo label-free optical monitoring of structural and metabolic remodeling of myocardium following infarction.Biomedical optics express · 2019Article
- Multispectral Depth-Resolved Fluorescence Lifetime Spectroscopy Using SPAD Array Detectors and Fiber Probes.Sensors (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Autofluorescence spectroscopy is a promising label-free approach to characterize biological samples with demonstrated potential to report structural and biochemical alterations in tissues in a number of clinical applications. We report a characterization of the ex vivo autofluorescence fingerprint of cardiac tissue, exploiting a Langendorff-perfused isolated rat heart model to induce physiological insults to the heart, with a view to understanding how metabolic alterations affect the autofluorescence signals. Changes in the autofluorescence intensity and lifetime signatures associated with reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) were characterized during oxygen- or glucose-depletion protocols. Results suggest that both NAD(P)H and FAD autofluorescence intensity and lifetime parameters are sensitive to changes in the metabolic state of the heart owing to oxygen deprivation. We also observed changes in NAD(P)H fluorescence intensity and FAD lifetime parameter on reperfusion of oxygen, which might provide information on reperfusion injury, and permanent tissue damage or changes to the tissue during recovery from oxygen deprivation. We found that changes in the autofluorescence signature following glucose-depletion are, in general, less pronounced, and most clearly visible in NAD(P)H related parameters. Overall, the results reported in this investigation can serve as baseline for future investigations of cardiac tissue involving autofluorescence measurements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.