ArticleStem cells and development2018
FACT Inhibition Blocks Induction But Not Maintenance of Pluripotency.
Article in Stem cells and development, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Beyond Chaperoning: The Multifaceted Role of FACT in Chromatin Transactions.International journal of molecular sciences · 2025Review
- Oct4 redox sensitivity potentiates reprogramming and differentiation.Genes & development · 2024Article
- Pluripotency state transition of embryonic stem cells requires the turnover of histone chaperone FACT on chromatin.iScience · 2024Article
- Transcriptional regulation of FACT involves Coordination of chromatin accessibility and CTCF binding.The Journal of biological chemistry · 2024Article
- FACT regulates pluripotency through proximal and distal regulation of gene expression in murine embryonic stem cells.BMC biology · 2023Article
- Article
- Silencing ofInsects · 2022Article
- Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.ACS applied materials & interfaces · 2022Article
- Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.Communications biology · 2022Article
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- The role of FACT in managing chromatin: disruption, assembly, or repair?Nucleic acids research · 2020Review
- Partial Replacement of Nucleosomal DNA with Human FACT Induces Dynamic Exposure and Acetylation of Histone H3 N-Terminal Tails.iScience · 2020Article
- Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters.Nucleic acids research · 2020Article
- Histone chaperone FACT is essential to overcome replication stress in mammalian cells.Oncogene · 2020Article
- Chromatin regulation and dynamics in stem cells.Current topics in developmental biology · 2020Review
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- Histone Chaperone FACT and Curaxins: Effects on Genome Structure and Function.Journal of cancer metastasis and treatment · 2019Article
- Chaperones and Beyond as Key Players in Pluripotency Maintenance.Frontiers in cell and developmental biology · 2019Review
- Regulation of chromatin structure and function: insights into the histone chaperone FACT.Cell cycle (Georgetown, Tex.)Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The histone chaperone facilitates chromatin transactions (FACT) is associated with nuclear processes, including DNA transcription, replication, and repair. We previously showed that FACT is transiently recruited to pluripotency-associated target genes by newly bound Oct4. In this study, we tested the effects of FACT depletion by knockout or chemical inhibition on the induction and maintenance of pluripotency. Clustered regularly interspaced short palindromic repeat (CRISPR)-mediated deletion of the FACT subunit Spt16 did not affect the viability or proliferation of fibroblasts but blocked their ability to form induced pluripotent stem cells. Similarly, a small molecule inhibitor of FACT blocked the induction of pluripotency at an early step in reprogramming, without affecting the viability, proliferation, undifferentiated state, or the expression of core pluripotency genes. Notably, trypsinization and passage of pluripotent cells transiently reintroduced a requirement for FACT. Although FACT has been considered to be an essential transcription elongation factor, these results contribute to the emerging view that it instead promotes transitions between stable chromatin states, including during reprogramming to pluripotency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.