Evidence map›Paper›PMID 30318302›Full record

ArticleCell stem cell2018

CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming.

Yanxia Liu, Chen Yu, Timothy Patrick Daley, Fangyuan Wang, William S Cao, Salil Bhate, Xueqiu Lin, Chris Still, Honglei Liu, Dehua Zhao and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell stem cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 140 papers.

0numbers the graph read from it
0cells of the map it votes in
140citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

140 citing papers in PubMed.

  1. Review
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  14. Direct in vivo reprogramming to relieve tissue ischemia via induced vasculogenesis.Molecular therapy : the journal of the American Society of Gene Therapy · 2025
    Review
  15. Article
  16. Cutting-edge technologies in neural regeneration.Cell regeneration (London, England) · 2025
    Review
  17. Article
  18. Human Synthetic Biology and Programmable Gene Regulation Control.Annual review of genomics and human genetics · 2025
    Review
  19. Review
  20. Review

80 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors.

Yanxia LiuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Chen YuThe Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA.
Timothy Patrick DaleyDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Statistics & Department of Biomedical Data Science, Stanford University, Stanford, CA 94305, USA.
Fangyuan WangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Shanghai Institute of Rheumatology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
William S CaoAfaSci Research Laboratories, Redwood City, CA 94063, USA.
Salil BhateDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Xueqiu LinDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Chris StillGraduate Program of Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, CA 94305, USA.
Honglei LiuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Dehua ZhaoDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Haifeng WangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA.
Xinmin S XieAfaSci Research Laboratories, Redwood City, CA 94063, USA.
Sheng DingThe Gladstone Institute of Cardiovascular Disease, San Francisco, CA 94158, USA; School of Pharmaceutical Sciences, Tsinghua University, Beijing 100084, China.
Wing Hung WongDepartment of Statistics & Department of Biomedical Data Science, Stanford University, Stanford, CA 94305, USA.
Marius WernigDepartment of Pathology, Institute for Stem Cell Biology and Regenerative Medicine, Stanford, CA 94305, USA.
Lei S QiDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA. Electronic address: stanley.qi@stanford.edu.

Funding

A novel RNA-guided platform for sequence-specific cell reprogrammingDP5OD017887 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI QI, LEI STANLEY · 2013 to 2017
$2.0M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01DA047732 · NIDA · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2018 to 2019
$1.6M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01EB021240 · NIBIB · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2015 to 2017
$1.4M
NIBIB NIH HHS U01 EB021240NIDA NIH HHS U01 DA047732NIH HHS DP5 OD017887
6 · The paper itself

Abstract

Comprehensive identification of factors that can specify neuronal fate could provide valuable insights into lineage specification and reprogramming, but systematic interrogation of transcription factors, and their interactions with each other, has proven technically challenging. We developed a CRISPR activation (CRISPRa) approach to systematically identify regulators of neuronal-fate specification. We activated expression of all endogenous transcription factors and other regulators via a pooled CRISPRa screen in embryonic stem cells, revealing genes including epigenetic regulators such as Ezh2 that can induce neuronal fate. Systematic CRISPR-based activation of factor pairs allowed us to generate a genetic interaction map for neuronal differentiation, with confirmation of top individual and combinatorial hits as bona fide inducers of neuronal fate. Several factor pairs could directly reprogram fibroblasts into neurons, which shared similar transcriptional programs with endogenous neurons. This study provides an unbiased discovery approach for systematic identification of genes that drive cell-fate acquisition.

Indexed as

Gene EditingAnimalsCells, CulturedCellular ReprogrammingCRISPR-Cas SystemsFemaleMaleMiceMice, Inbred C57BLMutagenesis, Site-DirectedNeuronsRatsRats, Sprague-DawleyTranscription FactorsTranscription Factorscell-fate determinationCRISPRCRISPR activationCRISPRa screenDNA-binding factorgenetic interactionneuronal reprogrammingstem celltranscription factor

Identifiers

PMID30318302
PMCPMC6214761

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.