Evidence map›Paper›PMID 30318144›Full record

ArticleCell2018

CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization.

Haifeng Wang, Xiaoshu Xu, Cindy M Nguyen, Yanxia Liu, Yuchen Gao, Xueqiu Lin, Timothy Daley, Nathan H Kipniss, Marie La Russa, Lei S Qi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.

0numbers the graph read from it
0cells of the map it votes in
126citing papers in PubMed
9.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

126 citing papers in PubMed, 217 citations in OpenAlex.

  1. 3D Genome Engineering Using CRISPR/dCas Systems.International journal of molecular sciences · 2026
    Review
  2. Article
  3. Article
  4. Advances and clinical potential of epigenome editing.Cellular and molecular life sciences : CMLS · 2026
    Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Epigenome editing based treatment: Progresses and challenges.Molecular therapy : the journal of the American Society of Gene Therapy · 2026
    Review
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Review
  20. Article

66 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Haifeng WangDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Xiaoshu XuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Cindy M NguyenDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Yanxia LiuDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Yuchen GaoDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Cancer Biology Program, Stanford University, Stanford, CA 94305, USA.
Xueqiu LinDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Timothy DaleyDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Statistics, Stanford University, Stanford, CA 94305, USA.
Nathan H KipnissDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Marie La RussaDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Lei S QiDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Chemical and Systems Biology, Stanford University, Stanford, CA 94305, USA; Stanford ChEM-H, Stanford University, Stanford, CA 94305, USA. Electronic address: stanley.qi@stanford.edu.
Stanford University · US

Funding

PREDOCTORAL TRAINING IN BIOMEDICAL SCIENCEST32GM008568 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ANSEL, KARL MARK · 1995 to 2019
$6.3M
A novel RNA-guided platform for sequence-specific cell reprogrammingDP5OD017887 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI QI, LEI STANLEY · 2013 to 2017
$2.0M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01DA047732 · NIDA · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2018 to 2019
$1.6M
Hi-resolution dynamic imaging of chromosomes in single cells by combined CRISPR imaging and sequential FISHU01EB021240 · NIBIB · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI CAI, LONG, QI, LEI STANLEY · 2015 to 2017
$1.4M
NIBIB NIH HHS U01 EB021240NIDA NIH HHS U01 DA047732NIGMS NIH HHS T32 GM008568NIH HHS DP5 OD017887
6 · The paper itself

Abstract

Programmable control of spatial genome organization is a powerful approach for studying how nuclear structure affects gene regulation and cellular function. Here, we develop a versatile CRISPR-genome organization (CRISPR-GO) system that can efficiently control the spatial positioning of genomic loci relative to specific nuclear compartments, including the nuclear periphery, Cajal bodies, and promyelocytic leukemia (PML) bodies. CRISPR-GO is chemically inducible and reversible, enabling interrogation of real-time dynamics of chromatin interactions with nuclear compartments in living cells. Inducible repositioning of genomic loci to the nuclear periphery allows for dissection of mitosis-dependent and -independent relocalization events and also for interrogation of the relationship between gene position and gene expression. CRISPR-GO mediates rapid de novo formation of Cajal bodies at desired chromatin loci and causes significant repression of endogenous gene expression over long distances (30-600 kb). The CRISPR-GO system offers a programmable platform to investigate large-scale spatial genome organization and function.

Indexed as

GenomeAbscisic AcidCell Line, TumorChromatinCoiled BodiesCRISPR-Cas SystemsGene EditingGene Expression RegulationGenetic LociHumansIn Situ Hybridization, FluorescenceS Phase Cell Cycle CheckpointsAbscisic AcidChromatin3D genomeCajal bodyCas9CRISPRCRISPR-GOgene regulationgene repressionnuclear bodynuclear peripheryPML body

Identifiers

PMID30318144
PMCPMC6239909
OpenAlexW2897138703

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.