Evidence map›Paper›PMID 30317506›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Use of Surface Plasmon Resonance (SPR) to Determine Binding Affinities and Kinetic Parameters Between Components Important in Fusion Machinery.

Robert P Sparks, Jermaine L Jenkins, Rutilio Fratti

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 75 citations in OpenAlex.

  1. Article
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  9. EstablishingAnalytical chemistry · 2026
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  18. Binding affinity between coronavirus spike protein and human ACE2 receptor.Computational and structural biotechnology journal · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Robert P SparksDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Jermaine L JenkinsStructural Biology and Biophysics Facility, University of Rochester, Rochester, NY, USA.
Rutilio FrattiDepartment of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL, USA. rfratti@illinois.edu.
University of Illinois Urbana-Champaign · USUniversity of Rochester · US

Funding

Allosteric Regulation of SNAREs and the Rab GEF Mon1 by PhosphoinositidesR01GM101132 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI FRATTI, RUTILIO A · 2012 to 2016
$1.4M
NIGMS NIH HHS R01 GM101132
6 · The paper itself

Abstract

Surface plasmon resonance (SPR) can be used to analyze both binding affinities and kinetic parameters between a ligand and an analyte. SPR can be performed by either cross-linking a given ligand to a sensor chip covalently or utilizing high-affinity non-covalent interactions to secure a ligand in a particular conformation to a chip, both of which have their potential advantages. SPR measurements are mass based and reflect the proportional amount of analyte bound to a given ligand at a given concentration when flowed at a set rate in order to determine the binding parameters of a given biochemical interaction. The resultant sensorgram can indicate different types of binding events as well as provide both k

Indexed as

Protein BindingKineticsLigandsLipidsMembrane FusionNanostructuresN-Ethylmaleimide-Sensitive ProteinsSNARE ProteinsSoluble N-Ethylmaleimide-Sensitive Factor Attachment ProteinsSurface Plasmon ResonanceLigandsLipidsN-Ethylmaleimide-Sensitive ProteinsSNARE ProteinsSoluble N-Ethylmaleimide-Sensitive Factor Attachment ProteinsNanodiscNSFPhosphatidic acidSec17Sec18SNAREα-SNAP

Identifiers

PMID30317506
PMCPMC8489108
OpenAlexW2896617527

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.