Evidence map›Paper›PMID 30315421›Full record

ArticleClinical oral investigations2019

Dental pulp stem cells overexpressing stromal-derived factor-1α and vascular endothelial growth factor in dental pulp regeneration.

Lifang Zhu, Waruna Lakmal Dissanayaka, Chengfei Zhang

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
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  8. Human dental pulp stem cells for spinal cord injury.Stem cell research & therapy · 2025
    Review
  9. Angiogenic regulation of dental pulp stem cells.Frontiers in dental medicine · 2025
    Review
  10. Article
  11. Article
  12. Review
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  15. Review
  16. Review
  17. Article
  18. Article
  19. Influencing factors of pulp-dentin complex regeneration and related biological strategies.Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2022
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Lifang ZhuEndodontology, Faculty of Dentistry, The University of Hong Kong, 3A15, Prince Philip Dental Hospital, 34, Hospital Road, Hong Kong, SAR, China.
Waruna Lakmal DissanayakaApplied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong, SAR, China.
Chengfei ZhangEndodontology, Faculty of Dentistry, The University of Hong Kong, 3A15, Prince Philip Dental Hospital, 34, Hospital Road, Hong Kong, SAR, China. zhangcf@hku.hk.
University of Hong Kong · HKChinese University of Hong Kong · CN

Funding

Research Grants Council, University Grants Committee GRF 17126914Research Grants Council, University Grants Committee HKU 784912
6 · The paper itself

Abstract

objectivesThe current study aimed to investigate the effects of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) overexpressing dental pulp stem cells (DPSCs) in vascularized dental pulp regeneration in vivo. MATERIALS AND

methodsHuman DPSCs were transfected with VEGF or SDF-1α using premade lentiviral particles. Overexpression was verified by quantitative polymerase chain reaction (q-PCR), enzyme-linked immunosorbent assay (ELISA), and western blot analysis. Effects of SDF-1α and VEGF overexpressing DPSCs on their proliferation (CCK-8 and MTT assays) and endothelial vascular-tube formation (Matrigel assay) were investigated in vitro. Human tooth roots sectioned into 6-mm segments were injected with gene-modified DPSCs encapsulated in PuraMatrix hydrogel and implanted in the dorsum of severe-combined-immunodeficient (SCID) mice. Implants were retrieved after 4 weeks and examined for regenerated pulp-like tissue and vascularization using histology and immunohistochemistry. p < 0.05 was considered statistically significant.

resultsGene-modified DPSCs expressed significantly high levels (p < 0.05) of SDF-1α and VEGF mRNA and proteins, respectively. Transfected DPSCs showed a significantly higher cell proliferation compared to that of wild-type DPSCs. Furthermore, they enhanced endothelial cell migration and vascular-tube formation on Matrigel in vitro. When injected into tooth root canals and implanted in vivo, DPSCs/SDF-1α + DPSCs/VEGF-mixed group resulted in significantly increased length of regenerated pulp-like tissue within the root canals compared to that of wild-type DPSCs/VEGF and DPSCs/SDF-1α groups. Vessel area density was significantly higher in DPSCs/SDF-1α and mixed DPSCs/SDF-1α + DPSCs/VEGF groups than in DPSCs-VEGF alone or wild-type DPSCs groups.

conclusionA combination of VEGF-overexpressing and SDF-1α-overexpressing DPSCs could enhance the area of vascularized dental pulp regeneration in vivo. CLINICAL RELEVANCE: Enhancing vascularization in pulp regeneration is crucial to overcome the clinical limitation of the limited blood supply to the root canals via a small apical foramen enclosed by hard dentin.

Indexed as

RegenerationAnimalsCell DifferentiationCell ProliferationCells, CulturedChemokine CXCL12Dental PulpFemaleHumansHuman Umbilical Vein Endothelial CellsMiceMice, SCIDStem CellsTransfectionVascular Endothelial Growth Factor AChemokine CXCL12CXCL12 protein, humanVascular Endothelial Growth Factor AVEGFA protein, humanAngiogenesisDental pulp stem cellsPulp regenerationStromal derived factor-1αVascular endothelial growth factor

Identifiers

PMID30315421
OpenAlexW2897538431

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.