ArticleBMC infectious diseases2018
Modified genome comparison method: a new approach for identification of specific targets in molecular diagnostic tests using Mycobacterium tuberculosis complex as an example.
Article in BMC infectious diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed.
- Novel Target for Pathogenic Leptospira Detection via Colorimetric Loop-Mediated Isothermal Amplification (LAMP): Development, Validation, and Comparative Analysis.Journal of clinical laboratory analysis · 2026Article
- Article
- Status and outlook of pulmonary tuberculosis coinfection.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2025Review
- Impact ofMicroorganisms · 2024Article
- COVID-19 target: A specific target for novel coronavirus detection.Gene reports · 2020Article
- Targeting novel genes for simultaneous detection of five fungal and bacterial agents from BAL samples using multiplex PCR assay.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Genotypic and phenotypic characterization of Mycobacterium tuberculosis resistance against fluoroquinolones in the northeast of Iran.BMC infectious diseases · 2020Article
- Development of a Cost-Effective Line Probe Assay for Rapid Detection and Differentiation ofReports of biochemistry & molecular biology · 2020Article
- A New Specific DNA Target Sequence for Identification ofAvicenna journal of medical biotechnologyArticle
- Identification of a Novel Nucleic Acid Target for the Rapid and Specific Detection ofAvicenna journal of medical biotechnologyArticle
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7 authors.
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Abstract
backgroundThe first step of designing any genome-based molecular diagnostic test is to find a specific target sequence. The modified genome comparison method is one of the easiest and most comprehensive ways to achieve this goal. In this study, we aimed to explain this method with the example of Mycobacterium tuberculosis complex and investigate its efficacy in a diagnostic test.
methodsA specific target was identified using modified genome comparison method and an in-house PCR test was designed. To determine the analytical sensitivity and specificity, 10 standard specimens were used. Also, 230 specimens were used to determine the clinical sensitivity and specificity.
resultsThe identity and query cover of our new diagnostic target (5KST) were ≥ 90% with M. tuberculosis complex. The 5KST-PCR sensitivity was 100% for smear-positive, culture-positive and 85.7% for smear-negative, culture-positive specimens. All of 100 smear-negative, culture-negative specimens were negative in 5KST-PCR (100% clinical specificity). Analytical sensitivity of 5KST-PCR was approximately 1 copy of genomic DNA per microliter.
conclusionsModified genome comparison method is a confident way to find specific targets for use in diagnostic tests. Accordingly, the 5KST-PCR designed in this study has high sensitivity and specificity and can be replaced for conventional TB PCR tests.
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