ArticleTissue engineering. Part A2019
Human Testis Extracellular Matrix Enhances Human Spermatogonial Stem Cell Survival
Article in Tissue engineering. Part A, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 49 citations in OpenAlex.
- Modular 3D culture platform combining methylcellulose, testicular ECM, and Sertoli cells forIranian journal of basic medical sciences · 2026Article
- Fabrication of testicular decellularized scaffolds using a hybrid SDS-supercritical CO₂ method: process optimization, extracellular matrix preservation, and biocompatibility evaluation.Biomedical engineering online · 2025Article
- Effect of Extracellular Matrix Derived from Porcine Tissue on Stemness of Porcine Spermatogonial Stem Cells.International journal of molecular sciences · 2025Article
- TKTL1: a new candidate gene in non-obstructive azoospermia.Reproductive biomedicine online · 2025Article
- Male fertility restoration with stem cell-based therapies: from testicular stem cells to pluripotent stem cell-based in vitro gametogenesis.Fertility and sterility · 2025Review
- Chromolaena Odorata and male fertility: Evidence from the testes and epididymis of Wistar Rats.Reproduction & fertility · 2025Article
- Effectiveness of Voytik-Harbin Protocol in Fabrication of Ram's Testicular-Derived Hydrogel and Its Impact on MouseInternational journal of fertility & sterility · 2025Article
- Microenvironment of spermatogonial stem cells: a key factor in the regulation of spermatogenesis.Stem cell research & therapy · 2024Review
- Optimized Recovery of Immature Germ Cells after Prepubertal Testicular Tissue Digestion and Multi-Step Differential Plating: A Step towards Fertility Restoration with Cancer-Cell-Contaminated Tissue.International journal of molecular sciences · 2023Article
- In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.Cell and tissue research · 2023Review
- The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.International journal of fertility & sterility · 2023Article
- Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis.Stem cell research & therapy · 2023Review
- Dissecting the spermatogonial stem cell niche using spatial transcriptomics.Cell reports · 2023Article
- Favorable culture conditions for spermatogonial propagation in human and non-human primate primary testicular cell cultures: a systematic review and meta-analysis.Frontiers in cell and developmental biology · 2023Article
- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Impact of the hypoxic microenvironment on spermatogonial stem cells in culture.Frontiers in cell and developmental biology · 2023Article
- Current status and reflections on fertility preservation in China.Journal of assisted reproduction and genetics · 2022Review
- Male fertility preservation and restoration strategies for patients undergoing gonadotoxic therapies†.Biology of reproduction · 2022Review
- Effect of NMDA receptor agonist and antagonist on spermatogonial stem cells proliferation in 2- and 3- dimensional culture systems.Molecular biology reports · 2022Article
- Fertility preservation in boys facing gonadotoxic cancer therapy.Nature reviews. Urology · 2022Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
impact statementThis study developed and characterized human testis extracellular matrix (htECM) and porcine testis ECM (ptECM) for testing in human spermatogonial stem cell (hSSC) culture. Results confirmed the hypothesis that ECM from the homologous species (human) and homologous tissue (testis) is optimal for maintaining hSSCs. We describe a simplified feeder-free, serum-free condition for future iterative testing to achieve the long-term goal of stable hSSC cultures. To facilitate analysis and understand the fate of hSSCs in culture, we describe a multiparameter, high-throughput, quantitative flow cytometry approach to rapidly count undifferentiated spermatogonia, differentiated spermatogonia, apoptotic spermatogonia, and proliferative spermatogonia in hSSC cultures.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.