Evidence map›Paper›PMID 30311449›Full record

ArticleMicrobiologyOpen2019

A new strategy for seamless gene editing and marker recycling in Saccharomyces cerevisiae using lethal effect of Cwp1.

Yuxiao Hu, Yanrong Jia, Xiangdong Zhao, Zihao Yang, Zhimin Hao, Jingao Dong, Fanli Zeng

Open access · goldAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yuxiao HuCollege of Life Sciences, Hebei Agricultural University, Baoding, China.
Yanrong JiaCollege of Life Sciences, Hebei Agricultural University, Baoding, China.
Xiangdong ZhaoCollege of Life Sciences, Hebei Agricultural University, Baoding, China.
Zihao YangCollege of Life Sciences, Hebei Agricultural University, Baoding, China.
Zhimin HaoCollege of Life Sciences, Hebei Agricultural University, Baoding, China.
Jingao DongCollege of Life Sciences, Hebei Agricultural University, Baoding, China.
Fanli ZengCollege of Life Sciences, Hebei Agricultural University, Baoding, China.ORCID 0000-0003-2241-1890
Hebei Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Technologies development for seamless gene editing and marker recycling has allowed frequent genomic engineering in Saccharomyces cerevisiae for desired laboratory strains and cell factory. Alternative new approaches are still required for complicated scenarios. In this study, we report that inducible overexpression of cell wall protein 1 (Cwp1) by galactose addition confers yeast cells a robust growth inhibition. Direct repeats flanking the Gal-CWP1:selectable marker cassette allow for its homology recombination excision and counter selection upon galactose addition, therefore enable seamless gene editing and marker recycling. We used this strategy and efficiently generated scarless Ade8 deletion mutants. Our results highlight the utility of lethal effect of Cwp1 overexpression a new counter selection strategy and a simple and efficient method for seamless gene editing and marker recycling in S. cerevisiae and potentially other fungi.

Indexed as

GalactoseGene DeletionGene EditingHomologous RecombinationMembrane GlycoproteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCWP1 protein, S cerevisiaeGalactoseMembrane GlycoproteinsSaccharomyces cerevisiae ProteinsCwp1gene deletionmarker recyclingSaccharomyces cerevisiaeseamless gene editing

Identifiers

PMID30311449
PMCPMC6562115
OpenAlexW2896386199

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.