Evidence map›Paper›PMID 30310994›Full record

ArticleCurrent genetics2019

Proteomic profiling of yeast heterochromatin connects direct physical and genetic interactions.

Alexis Zukowski, Juliana Phillips, Soyeon Park, Ronghu Wu, Steven P Gygi, Aaron M Johnson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. The ATAD2/ANCCA homolog Yta7 cooperates with Scm3Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Alexis ZukowskiDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, 12801 E. 17th Ave, Aurora, CO, 80045, USA.
Juliana PhillipsDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, 12801 E. 17th Ave, Aurora, CO, 80045, USA.
Soyeon ParkDepartment of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Boulder, CO, 80309, USA.
Ronghu WuDepartment of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
Steven P GygiDepartment of Cell Biology, Harvard Medical School, Boston, MA, 02115, USA.
Aaron M JohnsonDepartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, 12801 E. 17th Ave, Aurora, CO, 80045, USA. aaron.m.johnson@ucdenver.edu.ORCID http://orcid.org/0000-0003-4553-1078
University of Colorado Anschutz Medical Campus · USGeorgia Institute of Technology · USHarvard University · USUniversity of Colorado Boulder · US

Funding

New Sample Multiplexing Technologies to Identify Chemical Probes and Illuminate Ubiquitin BiologyR01GM067945 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI GYGI, STEVEN P · 2003 to 2024
$10.5M
Predoctoral Training Program in Molecular BiologyT32GM008730 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI PREKERIS, RYTIS, SCLAFANI, ROBERT A · 1999 to 2019
$3.3M
New Technologies for Quantitative Phosphorylation AnalysisR01HG003456 · NHGRI · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI GYGI, STEVEN P · 2005 to 2011
$2.7M
Mechanisms of heterochromatin targeting and epigenetic genome regulationR35GM119575 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI JOHNSON, AARON M. · 2016 to 2020
$1.9M
Mechanisms of Chaperone-Mediated Control in the Assembly of the Proteasome HoloenzymeR01GM127688 · NIGMS · UNIVERSITY OF COLORADO · PI PARK, SOYEON · 2018 to 2022
$1.6M
Comprehensive Characterization of Heterochromatin DomainsR00GM094291 · NIGMS · UNIVERSITY OF COLORADO DENVER · PI JOHNSON, AARON M. · 2012 to 2014
$728k
NHGRI NIH HHS NIH HG3456NHGRI NIH HHS R01 HG003456NIGMS NIH HHS GM67945NIGMS NIH HHS R00 GM094291NIGMS NIH HHS R00GM094291NIGMS NIH HHS R01 GM067945NIGMS NIH HHS R01 GM127688NIGMS NIH HHS R35 GM119575NIGMS NIH HHS R35GM119575NIGMS NIH HHS T32 GM008730NIGMS NIH HHS T32GM008730
6 · The paper itself

Abstract

Heterochromatin domains are stably repressed chromatin structures composed of a core assembly of silencing proteins that condense adjacent nucleosomes. The minimal heterochromatin structure can serve as a platform for recruitment of complementary regulatory factors. We find that a reconstituted budding yeast heterochromatin domain can act as a platform to recruit multiple factors that play a role in regulating heterochromatin function. We uncover the direct interaction between the SIR heterochromatin complex and a chromosomal boundary protein that restricts the spread of heterochromatin. We find that the SIR complex relieves a mechanism of auto-inhibition within the boundary protein Yta7, allowing the Yta7 bromodomain to engage chromatin. Our results suggest that budding yeast shares with other eukaryotes the ability to establish complex heterochromatin domains that coordinate multiple mechanisms of silencing regulation through physical interactions.

Indexed as

Epistasis, GeneticProteomicsGene Expression Regulation, FungalGene SilencingGenes, ReporterGenetic VectorsHeterochromatinMass SpectrometryModels, BiologicalProteomeReproducibility of ResultsYeastsHeterochromatinProteomeHeterochromatinProteasomeSIR complexYta7

Identifiers

PMID30310994
PMCPMC6421107
OpenAlexW2896171396

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.