ArticleChemical science2018
A new structure-activity relationship for cyanine dyes to improve photostability and fluorescence properties for live cell imaging.
Article in Chemical science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Fluorogenic Sydnones for Bioorthogonal Labeling of DNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Single-Molecule Chemistry Part II: Pathway Analysis of the Oxidation of Guanine to 8-Oxo-7,8-dihydroguanosine in an Oligonucleotide Hybrid.Molecules (Basel, Switzerland) · 2026Article
- Combined mass spectrometry and computational analysis of cyanine dyes and their structural analogues.RSC advances · 2026Article
- Red light excitation: illuminating photocatalysis in a new spectrum.Beilstein journal of organic chemistry · 2025Review
- Near-infrared photocatalysis with cyanines: synthesis, applications and perspectives.Chemical science · 2024Review
- The Efficiency of Metabolic Labeling of DNA by Diels-Alder Reactions with Inverse Electron Demand: Correlation with the Size of Modified 2'-Deoxyuridines.ACS chemical biology · 2023Article
- Two-Factor Fluorogenicity of Tetrazine-Modified Cyanine-Styryl Dyes for Bioorthogonal Labelling of DNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Cationic styryl dyes for DNA labelling and selectivity toward cancer cells and Gram-negative bacteria.RSC advances · 2023Article
- Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.Bioconjugate chemistry · 2022Article
- Article
- Selective, pH-Dependent Colorimetric and Fluorimetric Detection of Quadruplex DNA with 4-Dimethylamino(phenyl)-Substituted Berberine Derivatives.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Cyanine Dyes Containing Quinoline Moieties: History, Synthesis, Optical Properties, and Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Review
- Bioinspired Heterocyclic Partnership in a Cyanine-Type Acidichromic Chromophore.Molecules (Basel, Switzerland) · 2020Article
- Identification of optimal fluorescent probes for G-quadruplex nucleic acids through systematic exploration of mono- and distyryl dye libraries.Beilstein journal of organic chemistry · 2019Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new set of cyanine-indole dyes was synthesized, characterized by optical and cytotoxic properties and subsequently applied for live cell imaging. Furthermore, these dyes were postsynthetically linked covalently to the 2'-position of uridine anchors in presynthesized oligonucleotides using the copper(i)-catalyzed azide-alkyne cycloaddition in order to evaluate their photostability and imaging properties in living cells. The nucleophilicity at position C-2 of the indole part of the dyes was elucidated as key for a new structure-activity relationship that served as a rational guide to improve the photostability and optical properties of these green-emitting dyes for live cell imaging of nucleic acids. While the photostability rises exponentially with decreasing nucleophilicity, thermal bleaching experiments confirmed an opposite trend supposing that the superoxide radical anion is mainly responsible for the photobleaching of the dyes. Furthermore, the cytotoxicities of the dyes were tested in HeLa cells and moderate to low LD
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.