Evidence map›Paper›PMID 30310259›Full record

ArticleWorld journal of gastroenterology2018

Potential involvement of heat shock proteins in pancreatic-duodenal homeobox-1-mediated effects on the genesis of gastric cancer: A 2D gel-based proteomic study.

Juan Ma, Bei-Bei Wang, Xiao-Yan Ma, Wei-Ping Deng, Li-Shu Xu, Wei-Hong Sha

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Juan MaDepartment of Gastroenterology and Hepatology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangdong Geriatrics Institute, Guangzhou 510080, Guangdong Province, China.
Bei-Bei WangDepartment of Gastroenterology and Hepatology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangdong Geriatrics Institute, Guangzhou 510080, Guangdong Province, China.
Xiao-Yan MaForensic Identification Institute, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China.
Wei-Ping DengDepartment of Gastroenterology and Hepatology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangdong Geriatrics Institute, Guangzhou 510080, Guangdong Province, China.
Li-Shu XuDepartment of Gastroenterology and Hepatology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangdong Geriatrics Institute, Guangzhou 510080, Guangdong Province, China.
Wei-Hong ShaDepartment of Gastroenterology and Hepatology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangdong Geriatrics Institute, Guangzhou 510080, Guangdong Province, China. shaweihong@gdghospital.com.cn.
Guangzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo identify functional proteins involved in pancreatic-duodenal homeobox-1 (PDX1)-mediated effects on gastric carcinogenesis.

methodsA PDX1-overexpressed model was established by transfecting gastric cancer cell line SGC7901 with pcDNA3.1(+)-PDX1 vector (SGC-PDX1). Transfection with empty pcDNA3.1 vector (SGC-pcDNA) served as control. Comparative protein profiles of the two groups were analyzed by two-dimensional electrophoresis based-proteomics (2DE gel-based proteomics). The differential proteins identified by 2DE were further validated by qRT-PCR and immunoblotting. Finally, co-immunoprecipitation was used to determine any direct interactions between PDX1 and the differential proteins.

results2DE gel proteomics identified seven differential proteins in SGC-PDX1 when compared with those in SGC-pcDNA. These included four heat shock proteins (HSPs; HSP70p1B, HSP70p8, HSP60, HSP27) and three other proteins (ER60, laminin receptor 1, similar to epsilon isoform of 14-3-3 protein). Immunoblotting validated the expression of the HSPs (HSP70, HSP60, HSP27). Furthermore, their expressions were lowered to 80%, 20% and 24%, respectively, in SGC-PDX1, while PDX1 exhibited a 9-fold increase, compared to SGC-pcDNA. However, qRT-PCR analysis revealed that mRNA levels of the

conclusionThis study demonstrates the potential involvement of HSPs in PDX1-mediated effects on the genesis of gastric cancer.

Indexed as

Gene Expression Regulation, NeoplasticBiomarkers, TumorCell Line, TumorDown-RegulationElectrophoresis, Gel, Two-DimensionalGene Expression ProfilingGenes, HomeoboxHeat-Shock ProteinsHomeodomain ProteinsHSP70 Heat-Shock ProteinsHumansImage Processing, Computer-AssistedPancreasProtein Processing, Post-TranslationalProteomicsRNA, MessengerBiomarkers, TumorHeat-Shock ProteinsHomeodomain ProteinsHSP70 Heat-Shock Proteinspancreatic and duodenal homeobox 1 proteinRNA, MessengerTrans-ActivatorsGastric cancerHeat shock proteinsPancreatic-duodenal homeobox-1ProteomicsTwo-dimensional electrophoresis

Identifiers

PMID30310259
PMCPMC6175762
OpenAlexW2893575318

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.