ArticleScientific reports2018
Induction of the viable but non-culturable state in bacterial pathogens by household cleaners and inorganic salts.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 68 citations in OpenAlex.
- Novel molecular assessment method using precursor ribosomal RNA to rapidly quantify hard-to-monitor pathogens in aqueous suspensions: application to ultraviolet disinfection ofJournal of water and health · 2025Article
- Article
- Viable but non-cultivable state in oral microbiota: a critical review of an underexplored microbial survival strategy.Frontiers in cellular and infection microbiology · 2025Review
- Aquatic environment drives the emergence of cell wall-deficient dormant forms in Listeria.Nature communications · 2024Article
- Understanding the transition to viable but non-culturable state in Escherichia coli W3110: a comprehensive analysis of potential spectrochemical biomarkers.World journal of microbiology & biotechnology · 2024Article
- Biofilms as protective cocoons against biocides: from bacterial adaptation to One Health issues.Microbiology (Reading, England) · 2023Review
- Induction of Viable but Non-Culturable State in Clinically Relevant Staphylococci and Their Detection with Bacteriophage K.Antibiotics (Basel, Switzerland) · 2023Article
- Inaccuracies of the ISO 11731 Method for Environmental Validation ofMicroorganisms · 2022Article
- Effect of dilution factor on the isolation ofIranian journal of microbiology · 2022Article
- Overview of VBNC, a survival strategy for microorganisms.3 Biotech · 2022Review
- Taxonomical and functional bacterial community profiling in disease-resistant and disease-susceptible soybean cultivars.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2022Article
- It is complicated: Potential short- and long-term impact of coronavirus disease 2019 (COVID-19) on antimicrobial resistance-An expert review.Antimicrobial stewardship & healthcare epidemiology : ASHE · 2022Review
- The Viable But Non-Culturable State ofFrontiers in cellular and infection microbiology · 2022Review
- Formic acid, an organic acid food preservative, induces viable-but-non-culturable state, and triggers new Antimicrobial Resistance traits inFrontiers in microbiology · 2022Article
- Research progress in theZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2021Article
- The potential impact of the COVID-19 pandemic on global antimicrobial and biocide resistance: an AMR Insights global perspective.JAC-antimicrobial resistance · 2021Review
- Brief survey on organometalated antibacterial drugs and metal-based materials with antibacterial activity.RSC chemical biology · 2021Review
- Virulence Factors in Coagulase-Negative Staphylococci.Pathogens (Basel, Switzerland) · 2021Review
- How to Evaluate Non-Growing Cells-Current Strategies for Determining Antimicrobial Resistance of VBNC Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Direct Detection of Viable but Non-culturable (VBNC) Salmonella in Real Food System by a Rapid and Accurate PMA-CPA Technique.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effective monitoring of microbial pathogens is essential for a successful preventive food safety and hygiene strategy. However, as most monitoring strategies are growth-based, these tests fail to detect pathogenic bacteria that have entered the viable but non-culturable (VBNC) state. The present study reports the induction of the VBNC state in five human pathogens by commercially available household cleaners in combination with inorganic salts. We determined that non-ionic surfactants, a common ingredient in household cleaners, can induce the VBNC state, when combined with salts. A screening study with 630 surfactant/salt combinations indicates a correlation between the hydrophobicity of the surfactant and VBNC induction in L. monocytogenes, E. coli, S. enterica serovar Typhimurium, S. aureus and toxin-producing enteropathogenic E. coli. Cells that were exposed to combinations of surfactants and salts for 5 min and up to 1 h lost their culturability on standard growth media while retaining their ATP production, fermentation of sugars and membrane integrity, which suggests intact and active metabolism. Screening also revealed major differences between Gram-negative and Gram-positive bacteria; the latter being more susceptible to VBNC induction. Combinations of such detergents and salts are found in many different environments and reflect realistic conditions in industrial and domestic surroundings. VBNC cells present in industrial environments, food-processing plants and even our daily routine represent a serious health risk due to possible resuscitation, unknown spreading, production of toxins and especially their invisibility to routine detection methods, which rely on culturability of cells and fail to detect VBNC pathogens.
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