ArticleScientific reports2018
Antibiotic export by efflux pumps affects growth of neighboring bacteria.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Genome Analysis of 6222 Bacterial Isolates from Livestock and Food Environments in Spain to Decipher the Antibiotic Resistome.Antibiotics (Basel, Switzerland) · 2025Article
- Spatial population dynamics of bacterial colonies with social antibiotic resistance.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Advances in methods and concepts provide new insight into antibiotic fluxes across the bacterial membrane.Communications biology · 2024Article
- Antibiotic resistance in bacterial communities.Current opinion in microbiology · 2023Review
- Efflux, Signaling and Warfare in a Polymicrobial World.Antibiotics (Basel, Switzerland) · 2023Review
- Advances in linking single-cell bacterial stress response to population-level survival.Current opinion in biotechnology · 2023Review
- Monitoring lineages of growing and dividing bacteria reveals an inducible memory ofFrontiers in microbiology · 2023Article
- Calibrating spatiotemporal models of microbial communities to microscopy data: A review.PLoS computational biology · 2022Review
- MDR Pumps as Crossroads of Resistance: Antibiotics and Bacteriophages.Antibiotics (Basel, Switzerland) · 2022Review
- Antibiotics and ECMO in the Adult Population-Persistent Challenges and Practical Guides.Antibiotics (Basel, Switzerland) · 2022Review
- Multidrug Resistance Pumps as a Keystone of Bacterial Resistance.Moscow University biological sciences bulletin · 2022Article
- DeLTA 2.0: A deep learning pipeline for quantifying single-cell spatial and temporal dynamics.PLoS computational biology · 2022Article
- Effects of Incubation Time and Inoculation Level on the Stabilities of Bacteriostatic and Bactericidal Antibiotics againstAntibiotics (Basel, Switzerland) · 2021Article
- Cholic acid: a novel steroidal uncompetitive inhibitor against β-lactamase produced by multidrug-resistant isolates.World journal of microbiology & biotechnology · 2021Article
- Microscopic Approach to Intrinsic Antibiotic Resistance.The journal of physical chemistry. B · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cell-cell interactions play an important role in bacterial antibiotic resistance. Here, we asked whether neighbor proximity is sufficient to generate single-cell variation in antibiotic resistance due to local differences in antibiotic concentrations. To test this, we focused on multidrug efflux pumps because recent studies have revealed that expression of pumps is heterogeneous across populations. Efflux pumps can export antibiotics, leading to elevated resistance relative to cells with low or no pump expression. In this study, we co-cultured cells with and without AcrAB-TolC pump expression and used single-cell time-lapse microscopy to quantify growth rate as a function of a cell's neighbors. In inhibitory concentrations of chloramphenicol, we found that cells lacking functional efflux pumps (ΔacrB) grow more slowly when they are surrounded by cells with AcrAB-TolC pumps than when surrounded by ΔacrB cells. To help explain our experimental results, we developed an agent-based mathematical model, which demonstrates the impact of neighbors based on efflux efficiency. Our findings hold true for co-cultures of Escherichia coli with and without pump expression and also in co-cultures of E. coli and Salmonella typhumirium. These results show how drug export and local microenvironments play a key role in defining single-cell level antibiotic resistance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.