ArticlePlant physiology2019
Enzyme Fusion Removes Competition for Geranylgeranyl Diphosphate in Carotenogenesis.
Article in Plant physiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 86 citations in OpenAlex.
- Carotenoids in the Context of the Mediterranean Dietary Pattern: From Food Matrix and Bioavailability to Biomarkers and Human Health.Antioxidants (Basel, Switzerland) · 2026Review
- A metabolic complex of acyltransferase enzymes involved in tomato acylsugar biosynthesis.Journal of experimental botany · 2026Article
- Combining multiple metabolic strategies for efficient production of longifolene inSynthetic and systems biotechnology · 2026Article
- Suppression of microRNAs targeting key MEP pathway genes affects plastid terpenoid content and photosynthesis under variable light conditions.Molecular biology reports · 2026Article
- Family alliances feeding the carotenoid pathway in tomato.Journal of experimental botany · 2025Review
- Protein Prenylation Makeovers in Plants: Insights into Substrate Diversification.International journal of molecular sciences · 2025Review
- Beyond pigments and perfumes: engineering in the carotenoid and apocarotenoid spectrum, novel enzymes, and synthetic biology strategies.Frontiers in bioengineering and biotechnology · 2025Review
- Combining systems and synthetic biology for in vivo enzymology.The EMBO journal · 2024Article
- Nudix hydrolase 23 post-translationally regulates carotenoid biosynthesis in plants.The Plant cell · 2024Article
- Multidomain chimeric enzymes as a promising alternative for biocatalysts improvement: a minireview.Molecular biology reports · 2024Review
- Glucose 6-phosphate dehydrogenase variants increase NADPH pools for yeast isoprenoid production.FEBS open bio · 2024Article
- Comparative transcriptomic and plastid development analysis sheds light on the differential carotenoid accumulation in kiwifruit flesh.Frontiers in plant science · 2023Article
- Characterization of norbelladine synthase and noroxomaritidine/norcraugsodine reductase reveals a novel catalytic route for the biosynthesis of Amaryllidaceae alkaloids including the Alzheimer's drug galanthamine.Frontiers in plant science · 2023Article
- Mapping and Validation ofInternational journal of molecular sciences · 2022Article
- Design of Artificial Enzymes Bearing Several Active Centers: New Trends, Opportunities and Problems.International journal of molecular sciences · 2022Review
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Riboswitch-mediated inducible expression of an astaxanthin biosynthetic operon in plastids.Plant physiology · 2022Article
- Another level of complex-ity: The role of metabolic channeling and metabolons in plant terpenoid metabolism.Frontiers in plant science · 2022Review
- Phytoene Synthase: The Key Rate-Limiting Enzyme of Carotenoid Biosynthesis in Plants.Frontiers in plant science · 2022Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Geranylgeranyl diphosphate (GGPP), a prenyl diphosphate synthesized by GGPP synthase (GGPS), represents a metabolic hub for the synthesis of key isoprenoids, such as chlorophylls, tocopherols, phylloquinone, gibberellins, and carotenoids. Protein-protein interactions and the amphipathic nature of GGPP suggest metabolite channeling and/or competition for GGPP among enzymes that function in independent branches of the isoprenoid pathway. To investigate substrate conversion efficiency between the plastid-localized GGPS isoform GGPS11 and phytoene synthase (PSY), the first enzyme of the carotenoid pathway, we used recombinant enzymes and determined their in vitro properties. Efficient phytoene biosynthesis via PSY strictly depended on simultaneous GGPP supply via GGPS11. In contrast, PSY could not access freely diffusible GGPP or time-displaced GGPP supply via GGPS11, presumably due to liposomal sequestration. To optimize phytoene biosynthesis, we applied a synthetic biology approach and constructed a chimeric GGPS11-PSY metabolon (PYGG). PYGG converted GGPP to phytoene almost quantitatively in vitro and did not show the GGPP leakage typical of the individual enzymes.
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