Evidence map›Paper›PMID 30309388›Full record

ArticleStem cell research & therapy2018

Schwann cell-like differentiated adipose stem cells promote neurite outgrowth via secreted exosomes and RNA transfer.

Rosanna C Ching, Mikael Wiberg, Paul J Kingham

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
86citing papers in PubMed, 2 pooled it
7.2field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

86 citing papers in PubMed, 2 syntheses or guidelines pooled it, 141 citations in OpenAlex.

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  5. Peripheral nerve repair: innovations and future directions.Journal of translational medicine · 2026
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26 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Rosanna C ChingDepartment of Integrative Medical Biology, Section for Anatomy, Umeå University, 901 87, Umeå, Sweden.
Mikael WibergDepartment of Integrative Medical Biology, Section for Anatomy, Umeå University, 901 87, Umeå, Sweden.
Paul J KinghamDepartment of Integrative Medical Biology, Section for Anatomy, Umeå University, 901 87, Umeå, Sweden. paul.kingham@umu.se.
Umeå University · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAdipose derived stem cells can be stimulated to produce a growth factor rich secretome which enhances axon regeneration. In this study we investigated the importance of exosomes, extracellular vesicles released by many different cell types, including stem cells and endogenous nervous system Schwann cells (SCs), on neurite outgrowth.

methodsAdipose derived stem cells were differentiated towards a Schwann cell-like phenotype (dADSCs) by in vitro stimulation with a mix of factors (basic fibroblast growth factor, platelet derived growth factor-AA, neuregulin-1 and forskolin). Using a precipitation and low-speed centrifugation protocol the extracellular vesicles were isolated from the medium of the stem cells cultures and also from primary SCs. The conditioned media or concentrated vesicles were applied to neurons in vitro and computerised image analysis was used to assess neurite outgrowth. Total RNA was purified from the extracellular vesicles and investigated using qRT-PCR.

resultsApplication of exosomes derived from SCs significantly enhanced in vitro neurite outgrowth and this was replicated by the exosomes from dADSCs. qRT-PCR demonstrated that the exosomes contained mRNAs and miRNAs known to play a role in nerve regeneration and these molecules were up-regulated by the Schwann cell differentiation protocol. Transfer of fluorescently tagged exosomal RNA to neurons was detected and destruction of the RNA by UV-irradiation significantly reduced the dADSCs exosome effects on neurite outgrowth. In contrast, this process had no significant effect on the SCs-derived exosomes.

conclusionsIn summary, this work suggests that stem cell-derived exosomes might be a useful adjunct to other novel therapeutic interventions in nerve repair.

Indexed as

AdipocytesAdipose TissueAnimalsBiomarkersCell DifferentiationCoculture TechniquesCulture Media, ConditionedExosomesGAP-43 ProteinGene ExpressionIntercellular Signaling Peptides and ProteinsNerve RegenerationNeuritesNeuronal Outgrowthrac1 GTP-Binding ProteinRatsBiomarkersCulture Media, ConditionedGAP-43 ProteinIntercellular Signaling Peptides and ProteinsMapt protein, ratrac1 GTP-Binding ProteinRac1 protein, ratRhoA protein, ratrho GTP-Binding Proteinstau ProteinsExosomesExtracellular vesiclesPeripheral nervesRegenerationSchwann cellsStem cells

Identifiers

PMID30309388
PMCPMC6182785
OpenAlexW2896007618

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.