Evidence map›Paper›PMID 30308158›Full record

ArticleCell host & microbe2018

A Viral Protein Restricts Drosophila RNAi Immunity by Regulating Argonaute Activity and Stability.

Arabinda Nayak, Dong Young Kim, Michael J Trnka, Craig H Kerr, Peter V Lidsky, David J Stanley, Brianna Monique Rivera, Kathy H Li, Alma L Burlingame, Eric Jan and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell host & microbe, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 42 citations in OpenAlex.

  1. Article
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  5. Review
  6. Infection Defects of RNA and DNA Viruses Induced by Antiviral RNA Interference.Microbiology and molecular biology reviews : MMBR · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 3 countries.

Arabinda NayakDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94158, USA; Department of Biology and Genetics, Stanford University, Stanford, CA 94305, USA.
Dong Young KimDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA; College of Pharmacy, Yeungnam University, Gyeongsan, Gyeongbuk 38541, South Korea.
Michael J TrnkaDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Craig H KerrDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Peter V LidskyDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94158, USA.
David J StanleyDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Brianna Monique RiveraDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94158, USA.
Kathy H LiDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Alma L BurlingameDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Eric JanDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Judith FrydmanDepartment of Biology and Genetics, Stanford University, Stanford, CA 94305, USA.
John D GrossDepartment of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA 94158, USA.
Raul AndinoDepartment of Microbiology and Immunology, University of California San Francisco, San Francisco, CA 94158, USA. Electronic address: raul.andino@ucsf.edu.
University of California, San Francisco · USStanford University · USUniversity of British Columbia · CAYeungnam University · KR

Funding

X-ray Screening and Rapid Structure DeterminationP50GM082250 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI KROGAN, NEVAN J · 2007 to 2018
$48.8M
Unbiased Functional Characterization of Enterovirus-Host InteractionsP01AI091575 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ANDINO, RAUL · 2011 to 2015
$10.5M
Bio-Organic Biomedical Mass Spectromy ResourceP41GM103481 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BURLINGAME, ALMA L · 2012 to 2015
$6.4M
Mechanistic dissection of the link between virus infection and Alzheimer’s disease in a model organismR01AI137471 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ANDINO, RAUL · 2018 to 2022
$2.9M
Structure and Function of the Decapping Enzyme ComplexR01GM078360 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GROSS, JOHN D · 2009 to 2018
$2.5M
Defining the role of Host Hsp70 Subnetworks in Dengue Virus ReplicationR01AI127447 · NIAID · STANFORD UNIVERSITY · PI FRYDMAN, JUDITH · 2017 to 2021
$2.4M
Purchase of Q-Exactive Mass SpectrometerS10OD016229 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI BURLINGAME, ALMA L · 2013 to 2013
$507k
Howard Hughes Medical InstituteNIAID NIH HHS P01 AI091575NIAID NIH HHS R01 AI127447NIAID NIH HHS R01 AI137471NIGMS NIH HHS P41 GM103481NIGMS NIH HHS P50 GM082250NIGMS NIH HHS R01 GM078360NIH HHS S10 OD016229
6 · The paper itself

Abstract

The dicistrovirus, Cricket paralysis virus (CrPV) encodes an RNA interference (RNAi) suppressor, 1A, which modulates viral virulence. Using the Drosophila model, we combined structural, biochemical, and virological approaches to elucidate the strategies by which CrPV-1A restricts RNAi immunity. The atomic resolution structure of CrPV-1A uncovered a flexible loop that interacts with Argonaute 2 (Ago-2), thereby inhibiting Ago-2 endonuclease-dependent immunity. Mutations disrupting Ago-2 binding attenuates viral pathogenesis in wild-type but not Ago-2-deficient flies. CrPV-1A also contains a BC-box motif that enables the virus to hijack a host Cul2-Rbx1-EloBC ubiquitin ligase complex, which promotes Ago-2 degradation and virus replication. Our study uncovers a viral-based dual regulatory program that restricts antiviral immunity by direct interaction with and modulation of host proteins. While the direct inhibition of Ago-2 activity provides an efficient mechanism to establish infection, the recruitment of a ubiquitin ligase complex enables CrPV-1A to amplify Ago-2 inactivation to restrict further antiviral RNAi immunity.

Indexed as

AnimalsArgonaute ProteinsCell LineDicistroviridaeDrosophila melanogasterDrosophila ProteinsHumansMutationProtein BindingProtein ConformationProtein Interaction MapsRNA InterferenceUbiquitin-Protein LigasesViral ProteinsVirus ReplicationAGO2 protein, DrosophilaArgonaute ProteinsDrosophila ProteinsUbiquitin-Protein LigasesViral ProteinsAgo-2Ago-2 degradationantiviral immunityCrPVE3 ligaseinsectsRNAiRNAi suppressor

Identifiers

PMID30308158
PMCPMC6450077
OpenAlexW2896198768

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.