ArticlePLoS pathogens2018
Escherichia coli mediated resistance of Entamoeba histolytica to oxidative stress is triggered by oxaloacetate.
Article in PLoS pathogens, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Amino acid metabolism by pig intestinal bacteria and consequences for intestinal health and environment: current knowledge and future directions.Journal of animal science and biotechnology · 2026Review
- Review
- Microbiota-derived indole differentially shapes Entamoeba histolytica physiology and promotes host-compatible colonization.PLoS neglected tropical diseases · 2026Article
- Comparative gut microbial enzyme functions in human amoebiasis: Implications for host-microbe interactions and veterinary parasitic models.Open veterinary journal · 2026Article
- Recycling of Undigested Proteins Provided by the Host to the Large Intestine Microbiota: Implication for Intestinal Bacterial Anabolism, Growth, and Physiology.Microorganisms · 2025Review
- Nutritional Status and Fecal Microbiota in School Children from the Galapagos and the Andean Region.Microbial ecology · 2025Article
- Differential producibility analysis reveals drug-associated carbon and nitrogen metabolite expressions in Mycobacterium tuberculosis.The Journal of biological chemistry · 2025Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity.NPJ biofilms and microbiomes · 2023Article
- Amino Acid-Derived Bacterial Metabolites in the Colorectal Luminal Fluid: Effects on Microbial Communication, Metabolism, Physiology, and Growth.Microorganisms · 2023Review
- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
- Review
- Article
- Host Protective Mechanisms to Intestinal Amebiasis.Trends in parasitology · 2021Review
- Co- and polymicrobial infections in the gut mucosa: The host-microbiota-pathogen perspective.Cellular microbiology · 2021Review
- Natural Infection withmSphere · 2020Article
- Revisiting Drug Development Against the Neglected Tropical Disease, Amebiasis.Frontiers in cellular and infection microbiology · 2020Review
- Paradigms of Protist/Bacteria Symbioses Affecting Human Health:Frontiers in microbiology · 2020Review
- Infection-Induced Intestinal Dysbiosis Is Mediated by Macrophage Activation and Nitrate Production.mBio · 2019Article
- Oxygen induces the expression of invasion and stress response genes in the anaerobic salmon parasite Spironucleus salmonicida.BMC biology · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amebiasis, a global intestinal parasitic disease, is due to Entamoeba histolytica. This parasite, which feeds on bacteria in the large intestine of its human host, can trigger a strong inflammatory response upon invasion of the colonic mucosa. Whereas information about the mechanisms which are used by the parasite to cope with oxidative and nitrosative stresses during infection is available, knowledge about the contribution of bacteria to these mechanisms is lacking. In a recent study, we demonstrated that enteropathogenic Escherichia coli O55 protects E. histolytica against oxidative stress. Resin-assisted capture (RAC) of oxidized (OX) proteins coupled to mass spectrometry (OX-RAC) was used to investigate the oxidation status of cysteine residues in proteins present in E. histolytica trophozoites incubated with live or heat-killed E. coli O55 and then exposed to H2O2-mediated oxidative stress. We found that the redox proteome of E. histolytica exposed to heat-killed E. coli O55 is enriched with proteins involved in redox homeostasis, lipid metabolism, small molecule metabolism, carbohydrate derivative metabolism, and organonitrogen compound biosynthesis. In contrast, we found that proteins associated with redox homeostasis were the only OX-proteins that were enriched in E. histolytica trophozoites which were incubated with live E. coli O55. These data indicate that E. coli has a profound impact on the redox proteome of E. histolytica. Unexpectedly, some E. coli proteins were also co-identified with E. histolytica proteins by OX-RAC. We demonstrated that one of these proteins, E. coli malate dehydrogenase (EcMDH) and its product, oxaloacetate, are key elements of E. coli-mediated resistance of E. histolytica to oxidative stress and that oxaloacetate helps the parasite survive in the large intestine. We also provide evidence that the protective effect of oxaloacetate against oxidative stress extends to Caenorhabditis elegans.
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