ArticlePloS one2018
Thy1 transgenic mice expressing the red fluorescent calcium indicator jRGECO1a for neuronal population imaging in vivo.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 99 citations in OpenAlex.
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- Transcranial Calcium Macro-Imaging From the Auditory Cortex of Thy1-Cre-Driven GCaMP8 Transgenic Rats.Neuropsychopharmacology reports · 2026Article
- Microglia surveillance is directed toward neuron activation during sustained intracortical microstimulation.Journal of neural engineering · 2026Article
- Neural barcoding representing cortical spatiotemporal dynamics based on continuous-time Markov chains.Cell reports methods · 2026Article
- A Protocol for Microprism-Based Two-Photon Imaging Of The Lateral Cortex In The Mouse Inferior Colliculus.Current protocols · 2026Article
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- Mapping of neurovascular and neurometabolic couplings by multimodal optical imaging.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- Brain region-specific gain modulation of place cells by VIP neurons.Nature communications · 2025Article
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- Review of cocaine-induced brain vascular and cellular function changes measuredNeurophotonics · 2025Review
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- Distal activity patterns shape the spatial specificity of neurovascular coupling.Nature neuroscience · 2024Article
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- Auditory cortex conveys non-topographic sound localization signals to visual cortex.Nature communications · 2024Article
- Astrocytes modulate cerebral blood flow and neuronal response to cocaine in prefrontal cortex.Molecular psychiatry · 2024Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Calcium imaging is commonly used to measure the neural activity of large groups of neurons in mice. Genetically encoded calcium indicators (GECIs) can be delivered for this purpose using non-invasive genetic methods. Compared to viral gene transfer, transgenic targeting of GECIs provides stable long-term expression and obviates the need for invasive viral injections. Transgenic mice expressing the green GECI GCaMP6 are already widely used. Here we present the generation and characterization of transgenic mice expressing the sensitive red GECI jRGECO1a, driven by the Thy1 promoter. Four transgenic lines with different expression patterns showed sufficiently high expression for cellular in vivo imaging. We used two-photon microscopy to characterize visual responses of individual neurons in the visual cortex in vivo. The signal-to-noise ratio in transgenic mice was comparable to, or better than, mice transduced with adeno-associated virus. In addition, we show that Thy1-jRGECO1a transgenic mice are useful for transcranial population imaging and functional mapping using widefield fluorescence microscopy. We also demonstrate imaging of visual responses in retinal ganglion cells in vitro. Thy1-jRGECO1a transgenic mice are therefore a useful addition to the toolbox for imaging activity in intact neural networks.
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