ArticleCellular microbiology2019
Rhoptry neck protein 2 expressed in Plasmodium sporozoites plays a crucial role during invasion of mosquito salivary glands.
Article in Cellular microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Demystifying Plasmodium development within the Anopheles mosquito.Current opinion in microbiology · 2026Review
- Functional analyses of male-specific early-phosphorylated proteins reveal an intrinsically disordered protein required for microgamete release inFrontiers in cellular and infection microbiology · 2026Article
- A pleckstrin homology domain protein is involved in crystalloid formation inFrontiers in cellular and infection microbiology · 2026Article
- Article
- Identification of a novel rhoptry protein expressed predominantly inFrontiers in cellular and infection microbiology · 2025Article
- The Plasmodium circumsporozoite protein.Trends in parasitology · 2024Review
- Rhoptry neck protein 4 plays important roles duringmSphere · 2023Article
- The claudin-like apicomplexan microneme protein is required for gliding motility and infectivity of Plasmodium sporozoites.PLoS pathogens · 2023Article
- Novel systems to study vector-pathogen interactions in malaria.Frontiers in cellular and infection microbiology · 2023Review
- An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma.The EMBO journal · 2022Article
- Vaccine co-display of CSP and Pfs230 on liposomes targeting two Plasmodium falciparum differentiation stages.Communications biology · 2022Article
- Plasmodium sporozoite disintegration during skin passage limits malaria parasite transmission.EMBO reports · 2022Article
- The AMA1-RON complex drives Plasmodium sporozoite invasion in the mosquito and mammalian hosts.PLoS pathogens · 2022Article
- A toolbox for conditional control of gene expression in apicomplexan parasites.Molecular microbiology · 2022Review
- Gliding motility ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Plasmodium sporozoites on the move: Switching from cell traversal to productive invasion of hepatocytes.Molecular microbiology · 2021Review
- Identification, Mapping, and Genetic Diversity of Novel Conserved Cross-Species Epitopes of RhopH2 inFrontiers in cellular and infection microbiology · 2021Article
- Detection of the Rhoptry Neck Protein Complex inmSphere · 2020Article
- The dimerisable Cre recombinase allows conditional genome editing in the mosquito stages of Plasmodium berghei.PloS one · 2020Article
- Rhoptry neck protein 2 expressed in Plasmodium sporozoites plays a crucial role during invasion of mosquito salivary glands.Cellular microbiology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Malaria parasite transmission to humans is initiated by the inoculation of Plasmodium sporozoites into the skin by mosquitoes. Sporozoites develop within mosquito midgut oocysts, first invade the salivary glands of mosquitoes, and finally infect hepatocytes in mammals. The apical structure of sporozoites is conserved with the infective forms of other apicomplexan parasites that have secretory organelles, such as rhoptries and micronemes. Because some rhoptry proteins are crucial for Plasmodium merozoite infection of erythrocytes, we examined the roles of rhoptry proteins in sporozoites. Here, we demonstrate that rhoptry neck protein 2 (RON2) is also localized to rhoptries in sporozoites. To elucidate RON2 function in sporozoites, we applied a promoter swapping strategy to restrict ron2 transcription to the intraerythrocytic stage in the rodent malaria parasite, Plasmodium berghei. Ron2 knockdown sporozoites were severely impaired in their ability to invade salivary glands, via decreasing the attachment capacity to the substrate. This is the first rhoptry protein demonstrated to be involved in salivary gland invasion. In addition, ron2 knockdown sporozoites showed less infectivity to hepatocytes, possibly due to decreased attachment/gliding ability, indicating that parts of the parasite invasion machinery are conserved, but their contribution might differ among infective forms. Our sporozoite stage-specific knockdown system will help to facilitate understanding the comprehensive molecular mechanisms of parasite invasion of target cells.
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