ArticleJournal of veterinary internal medicine2018
Evaluation of cell culture-grown Bartonella antigens in immunofluorescent antibody assays for the serological diagnosis of bartonellosis in dogs.
Article in Journal of veterinary internal medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Development and validation of enzyme-linked immunosorbent assays for the serodiagnosis of canine bartonelloses.Journal of clinical microbiology · 2025Article
- Development of a multiplex serological assay to detect immunoreactivity against a novelMicrobiology spectrum · 2025Article
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- Prospective serological and molecular cross-sectional study focusing on Bartonella and other blood-borne organisms in cats from Catalonia (Spain).Parasites & vectors · 2022Article
- Serological evidence of exposure to Bartonella sp. in dogs with suspected vector-borne diseases, toxoplasmosis and neosporosis.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2022Article
- Bartonella spp. seroepidemiology and associations with clinicopathologic findings in dogs in the United States.Journal of veterinary internal medicine · 2022Article
- Using Proteomic Approaches to Unravel the Response ofFrontiers in cellular and infection microbiology · 2022Article
- SuspectedClinical case reports · 2021Article
- Comparison of Serological and Molecular Assays forPathogens (Basel, Switzerland) · 2021Article
- Antibiotic Susceptibility of Bartonella Grown in Different Culture Conditions.Pathogens (Basel, Switzerland) · 2021Article
- Exposure of Domestic Cats to Three ZoonoticPathogens (Basel, Switzerland) · 2021Article
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- Bartonella rochalimae, a newly recognized pathogen in dogs.Journal of veterinary internal medicine · 2020Article
- Validation of Bartonella henselae Western Immunoblotting for Serodiagnosis of Bartonelloses in Dogs.Journal of clinical microbiology · 2020Article
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- Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii.Journal of veterinary internal medicine · 2020Article
- Article
- Evaluation of cell culture-grown Bartonella antigens in immunofluorescent antibody assays for the serological diagnosis of bartonellosis in dogs.Journal of veterinary internal medicine · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBecause of poor sensitivity and questionable specificity of immunofluorescent antibody assays (IFAs), serological diagnosis of Bartonella species infections in dogs remains challenging. Despite limitations, IFA testing is the historical "gold standard" for Bartonella serodiagnosis in animals and humans. Because most diagnostic laboratories test against only 1 or 2 Bartonella spp., testing against a broader panel of Bartonella antigens may enhance diagnostic sensitivity and specificity.
objectiveTo evaluate the sensitivity and specificity of Bartonella IFA using 8 cell culture-grown Bartonella spp. isolates. ANIMALS: Archived serum samples from 34 Bartonella spp. naturally exposed, polymerase chain reaction (PCR)-positive dogs and from 26 PCR-negative and IFA-negative dogs.
methodsBartonella IFA sensitivity and specificity were assessed using cell culture-grown whole cell antigens derived from 3 Bartonella henselae (Bh) strains (Bh Houston 1, Bh San Antonio Type 2, Bh California 1), 3 Bartonella vinsonii subsp. berkhoffii genotypes (Bvb I, II, and III), Bartonella koehlerae (Bk), and Bartonella quintana (Bq).
resultsOnly 62% of 34 Bartonella spp. PCR-positive dogs were seroreactive to any of the 8 Bartonella IFA antigens, indicating low IFA sensitivity. PCR-positive dogs were most often IFA seroreactive to Bq (n = 15), to Bvb II (n = 13), or to both (n = 9) antigens. Of the 26 previously IFA-negative/PCR-negative dogs, 4 (15%) were seroreactive using the expanded antigen panel. CONCLUSION AND CLINICAL IMPORTANCE: Despite IFA testing of dogs against 8 different Bartonella isolates, IFA sensitivity remained poor, and specificity was only 85%. Development of a reliable serological assay is needed to facilitate the diagnosis of Bartonella infection in dogs.
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