Evidence map›Paper›PMID 30306293›Full record

ArticleGlycoconjugate journal2018

Purification, characterization and fine sugar specificity of a N-Acetylgalactosamine specific lectin from Adenia hondala.

Mamta Sharma, Prajna Hegde, Kavita Hiremath, Vishwanath Reddy H, A S Kamalanathan, Bale M Swamy, Shashikala R Inamdar

Abstract read
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In one paragraph

Article in Glycoconjugate journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Hemagglutinin from the Root Tuber ofTurkish journal of pharmaceutical sciences · 2024
    Article
  4. Article
  5. In Vitro Phytotherapeutic Properties of Aqueous ExtractedMolecules (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Mamta SharmaDepartment of Studies in Biochemistry, Karnatak University, Dharwad, 580003, India.
Prajna HegdeDepartment of Studies in Biochemistry, Karnatak University, Dharwad, 580003, India.
Kavita HiremathDepartment of Studies in Biochemistry, Karnatak University, Dharwad, 580003, India.
Vishwanath Reddy HDepartment of Studies in Biochemistry, Karnatak University, Dharwad, 580003, India.
A S KamalanathanCentre for Bioseparation Technology, VIT University, Vellore, 632014, India.
Bale M SwamyDepartment of Studies in Biochemistry, Karnatak University, Dharwad, 580003, India.
Shashikala R InamdarDepartment of Studies in Biochemistry, Karnatak University, Dharwad, 580003, India. srinamdar2009@gmail.com.
Karnatak University · INVellore Institute of Technology University · IN

Funding

Overall Priority Score P41GM103694 · NIGMS · EMORY UNIVERSITY · PI CUMMINGS, RICHARD D · 2013 to 2018
$6.9M
Protein-Glycan Interaction Resource of the CFGR24GM098791 · NIGMS · EMORY UNIVERSITY · PI CUMMINGS, RICHARD D, PAULSON, JAMES C · 2011 to 2013
$2.6M
NIGMS NIH HHS R24 GM098791
6 · The paper itself

Abstract

Plant lectins are gaining interest because of their interesting biological properties. Several Adenia species, that are being used in traditional medicine to treat many health ailments have shown presence of lectins or carbohydrate binding proteins. Here, we report the purification, characterization and biological significance of N-Acetyl galactosamine specific lectin from Adenia hondala (AHL) from Passifloraceae family. AHL was purified in a single step by affinity chromatography on asialofetuin Sepharose 4B column, characterized and its fine sugar specificity determined by glycan array analysis. AHL is human blood group non specific and also agglutinates rabbit erythrocytes. AHL is a glycoprotein with 12.5% of the carbohydrate, SDS-PAGE, MALDI-TOF-MS and ESI-MS analysis showed that AHL is a monomer of 31.6 kDa. AHL is devoid of DNase activity unlike other Ribosome inactivating proteins (RIPs). Glycan array analysis of AHL revealed its highest affinity for terminal lactosamine or polylactosamine of N- glycans, known to be over expressed in hepatocellular carcinoma and colon cancer. AHL showed strong binding to human hepatocellular carcinoma HepG2 cells with MFI of 59.1 expressing these glycans which was effectively blocked by 93.1% by asialofetuin. AHL showed dose and time dependent growth inhibitory effects on HepG2 cells with IC

Indexed as

AcetylgalactosamineAnimalsDeoxyribonucleasesHaptensHemagglutinationHep G2 CellsHumansLectinsMolecular WeightMonosaccharidesPassifloraceaePlant RootsPolysaccharidesSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationSugarsAcetylgalactosamineDeoxyribonucleasesHaptensLectinsMonosaccharidesPolysaccharidesSugarsAdenia hondala lectinGlycan microarrayHepG2 cellsN-AcetylgalactosaminePassifloraceaePoly-LacNAc

Identifiers

PMID30306293
OpenAlexW2901339671

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.