ArticlePlant methods2018
Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- CRISPR/Cas9-mediated editing of theGM crops & food · 2026Article
- Cloning and functional verification of endogenous U6 promoters for developing an efficient CRISPR/Cas9-mediated genome editing system in kenaf (Hibiscus cannabinus L.).BMC plant biology · 2026Article
- A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce.Plants (Basel, Switzerland) · 2026Article
- Identification of a cytokinin-responsive core promoter and interacting transcription factors of MtCKX1 in Medicago truncatula.BMC plant biology · 2026Article
- Cloning and functional verification of endogenous U6 promoters for developing an efficient CRISPR/Cas9-mediated genome editing system in kenaf (Hibiscus cannabinus L.).Research square · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Review
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- Silencing of disease susceptibility genes: an effective disease resistance strategy against fungal pathogens.Plant cell reports · 2025Review
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- An Optimized Editing Approach for Wheat Genes by Improving sgRNA Design and Transformation Strategies.International journal of molecular sciences · 2025Article
- A Truncated Endogenous U6 Promoter Enables High-Efficiency CRISPR Editing in Flax (Plants (Basel, Switzerland) · 2025Article
- Convenient, high-efficiency multiplex genome editing in autotetraploid alfalfa using endogenousaBIOTECH · 2025Article
- Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption.Journal, genetic engineering & biotechnology · 2025Article
- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- Development of an endogenous promoter-driven CRISPR/Cas9 system for genome editing inForestry research · 2025Article
- Identification of U6 Promoter and Establishment of Gene-Editing System inPlants (Basel, Switzerland) · 2024Article
- Comparative Analysis of Promoter Activity in Crassostrea gigas Embryos: Implications for Bivalve Gene Editing.Marine biotechnology (New York, N.Y.) · 2024Article
- Identification and map-based cloning of an EMS-induced mutation in wheat gene TaSP1 related to spike architecture.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Optimization of CRISPR-Cas9 system iniScience · 2024Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWhen developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or time-consuming. Cotton is an important crop for the production of fiber, oil, and biofuel. However, the cotton stable transformation is usually performed using
resultsIn this study, we provide a new system to evaluate and validate the efficiency of CRISPR/Cas9 cassettes in cotton using a transient expression system. By using this system, we could select the most effective CRISPR/Cas9 cassettes before the stable transformation. We have also optimized the existing cotton CRISPR/Cas9 system to achieve vastly improved mutagenesis efficiency by incorporating an endogenous
conclusionsThis study provides essential improvements to maximize CRISPR/Cas9-mediated mutation efficiency by reducing risk and workload for the application of CRISPR/Cas9 approaches in the targeted mutagenesis of cotton.
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