Evidence map›Paper›PMID 30305358›Full record

ReviewJournal of virology2019

Going the Distance: Optimizing RNA-Seq Strategies for Transcriptomic Analysis of Complex Viral Genomes.

Daniel P Depledge, Ian Mohr, Angus C Wilson

Open access · bronzeAbstract readReview
In one paragraph

Review in Journal of virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
7.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 45 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Nanopore Guided Annotation of Transcriptome Architectures.bioRxiv : the preprint server for biology · 2024
    Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
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  16. Human placental biology at single-cell resolution: a contemporaneous review.BJOG : an international journal of obstetrics and gynaecology · 2022
    Review
  17. Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Daniel P DepledgeDepartment of Microbiology, New York University School of Medicine, New York, New York, USA daniel.depledge@nyumc.org angus.wilson@med.nyu.edu.ORCID 0000-0002-4292-0599
Ian MohrDepartment of Microbiology, New York University School of Medicine, New York, New York, USA.
Angus C WilsonDepartment of Microbiology, New York University School of Medicine, New York, New York, USA daniel.depledge@nyumc.org angus.wilson@med.nyu.edu.ORCID 0000-0002-5016-4164
New York University · US

Funding

Control of Translation in Herpesvirus Infected Cells - Resubmission - 1R01GM056927 · NIGMS · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI Ian J Mohr · 1999 to 2026
$7.6M
Virus Host Interactions that Regulate Translation in Cells Infected with HSV-1R01AI073898 · NIAID · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI MOHR, IAN J · 2008 to 2020
$5.0M
Modeling herpes simplex virus latency in human neuronsR21AI130618 · NIAID · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI WILSON, ANGUS C · 2018 to 2019
$466k
NIAID NIH HHS R01 AI073898NIAID NIH HHS R21 AI130618NIGMS NIH HHS R01 GM056927
6 · The paper itself

Abstract

Transcriptome profiling has become routine in studies of many biological processes. However, the favored approaches such as short-read Illumina RNA sequencing are giving way to long-read sequencing platforms better suited to interrogating the complex transcriptomes typical of many RNA and DNA viruses. Here, we provide a guide-tailored to molecular virologists-to the ins and outs of viral transcriptome sequencing and discuss the strengths and weaknesses of the major RNA sequencing technologies as tools to analyze the abundance and diversity of the viral transcripts made during infection.

Indexed as

Computational BiologyGene Expression ProfilingGene Expression Regulation, ViralGenome, ViralHigh-Throughput Nucleotide SequencingNanoporesRNA VirusesSequence Analysis, RNASingle-Cell AnalysisminIONnanopore sequencingnext-generation sequencingPacBioRNA sequencingsingle-cell RNA-seqtranscriptomevirus

Identifiers

PMID30305358
PMCPMC6288342
OpenAlexW2903793577

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.