Limitations in the isolation and stimulation of splenic mononuclear cells in a dasyurid marsupial, Phascogale calura.

C Letendre, L J Young, J M Old

Open access · goldAbstract read

In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact, top 88% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Host-specificity assessment of feline alphaherpesvirus-1 derived immunocontraceptive candidates in non-feline models.Virology journal · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

C LetendreSchool of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW, 2751, Australia.

L J YoungSchool of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW, 2751, Australia.

J M OldSchool of Science and Health, Western Sydney University, Locked Bag 1797, Penrith, NSW, 2751, Australia. J.Old@westernsydney.edu.au.

Western Sydney University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveMarsupials suffer from an increasing number of stressors in this changing world. Functional studies are thus needed to broaden our understanding of the marsupial immune system. The red-tailed phascogale (Phascogale calura) is a small Australian marsupial previously used in descriptive immunological studies. Here, we aimed to develop functional assays by isolating and stimulating blood and spleen mononuclear cells in vitro.

resultsWhile peripheral blood mononuclear cell (PBMC) were relatively easy to isolate, only 10

Indexed as

AnimalsAustraliaCell SeparationFemaleLeukocytes, MononuclearMaleMarsupialiaPhytohemagglutininsPrimary Cell CultureSpleenPhytohemagglutininsCell cultureDasyuridMarsupialsMitogenMononuclear cellsPBMCPhytohemaglutininSpleen

Identifiers

PMID30305168

DOI10.1186/s13104-018-3824-5

PMCPMC6180634

OpenAlexW2897843459

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.