Evidence map›Paper›PMID 30305022›Full record

ArticleBMC plant biology2018

Cloning and characterization of the homoeologous genes for the Rec8-like meiotic cohesin in polyploid wheat.

Guojia Ma, Wei Zhang, Liwang Liu, Wun S Chao, Yong Qiang Gu, Lili Qi, Steven S Xu, Xiwen Cai

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Understanding and harnessing unreduced gametes for crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Review
  2. Review
  3. Engineering apomixis in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023
    Review
  4. Article
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Guojia MaDepartment of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
Wei ZhangDepartment of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
Liwang LiuDepartment of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA.
Wun S ChaoUSDA-ARS, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA.
Yong Qiang GuUSDA-ARS, Western Regional Research Center, Albany, CA, 94710, USA.
Lili QiUSDA-ARS, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA.
Steven S XuUSDA-ARS, Red River Valley Agricultural Research Center, Fargo, ND, 58102, USA.
Xiwen CaiDepartment of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA. xiwen.cai@ndsu.edu.ORCID http://orcid.org/0000-0002-3937-1074
Edward T. Schafer Agricultural Research Center · USNorth Dakota State University · USNanjing Agricultural University · CNWestern Regional Research Center · US

Funding

Directorate for Biological Sciences 0457356
6 · The paper itself

Abstract

backgroundMeiosis is a specialized cell division critical for gamete production in the sexual reproduction of eukaryotes. It ensures genome integrity and generates genetic variability as well. The Rec8-like cohesin is a cohesion protein essential for orderly chromosome segregation in meiotic cell division. The Rec8-like genes and cohesins have been cloned and characterized in diploid models, but not in polyploids. The present study aimed to clone the homoeologous genes (homoeoalleles) for Rec8-like cohesin in polyploid wheat, an important food crop for humans, and to characterize their structure and function under a polyploid condition.

resultsWe cloned two Rec8-like homoeoalleles from tetraploid wheat (TtRec8-A1 and TtRec8-B1) and one from hexaploid wheat (TaRec8-D1), and performed expression and functional analyses of the homoeoalleles. Also, we identified other two Rec8 homoeoalleles in hexaploid wheat (TaRec8-A1 and TaRec8-B1) and the one in Aegilops tauschii (AetRec8-D1) by referencing the DNA sequences of the Rec8 homoeoalleles cloned in this study. The coding DNA sequences (CDS) of these six Rec8 homoeoalleles are all 1,827 bp in length, encoding 608 amino acids. They differed from each other primarily in introns although single nucleotide polymorphisms were detected in CDS. Substantial difference was observed between the homoeoalleles from the subgenome B (TtRec8-B1 and TaRec8-B1) and those from the subgenomes A and D (TtRec8-A1, TaRec8-A1, and TaRec8-D1). TtRec8-A1 expressed dominantly over TtRec8-B1, but comparably to TaRec8-D1, in polyploid wheat. In addition, we developed the antibody against wheat Rec8 and used the antibody to detect Rec8 cohesin in the Western blotting and subcellular localization analyses.

conclusionsThe Rec8 homoeoalleles from the subgenomes A and D are transcriptionally more active than the one from the subgenome B in polyploid wheat. The structural variation and differential expression of the Rec8 homoeoalleles indicate a unique cross-genome coordination of the homoeologous genes in polyploid wheat, and imply the distinction of the wheat subgenome B from the subgenomes A and D in the origin and evolution.

Indexed as

AllelesCell Cycle ProteinsChromosomal Proteins, Non-HistoneChromosome MappingCloning, MolecularCohesinsExonsGene Expression Regulation, PlantHaploidyIntronsMeiosisPlant ProteinsPolyploidyTriticumCell Cycle ProteinsChromosomal Proteins, Non-HistoneCohesinsPlant ProteinsAnd wheatGene cloningHomoeoalleleMeiosisPolyploidRec8 cohesin

Identifiers

PMID30305022
PMCPMC6180652
OpenAlexW2896265128

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.