ArticleBMC genomics2018
Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Overcoming Barriers in Porcine SCNT: A Comprehensive Review of Developmental Challenges and Innovations.International journal of molecular sciences · 2026Review
- Overexpression of the pioneer transcription factor Nr5a2 promotes the development of mouse somatic cell nuclear transfer embryos.PLoS biology · 2026Article
- Quantitative modeling of mRNA degradation reveals tempo-dependent mRNA clearance in early embryos.Nucleic acids research · 2025Article
- Strategies for modeling aging and age-related diseases.npj aging · 2024Review
- Essential roles of the nucleolus during early embryonic development: a regulatory hub for chromatin organization.Open biology · 2024Article
- Early embryonic lethality of mice lacking POLD2.Molecular reproduction and development · 2023Article
- Nuclear Transfer Arrest Embryos Show Massive Dysregulation of Genes Involved in Transcription Pathways.International journal of molecular sciences · 2021Article
- Functions and Regulation of Endogenous Retrovirus Elements during Zygotic Genome Activation: Implications for Improving Somatic Cell Nuclear Transfer Efficiency.Biomolecules · 2021Review
- Review
- Article
- Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24aScience advances · 2020Article
- MCRS1 is essential for epiblast development during early mouse embryogenesis.Reproduction (Cambridge, England) · 2020Article
- Various nuclear reprogramming systems using egg and oocyte materials.The Journal of reproduction and development · 2019Review
- Endogenous Retroviruses Function as Gene Expression Regulatory Elements During Mammalian Pre-implantation Embryo Development.International journal of molecular sciences · 2019Review
- Long non-coding RNAs potentially function synergistically in the cellular reprogramming of SCNT embryos.BMC genomics · 2018Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundNuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries. However, certain deficiencies severely restrict the applications of this technology.
resultsUsing single-embryo RNA-seq, our study provides complete transcriptome blueprints of embryos generated by cumulus cell (CC) donor nuclear transfer (NT), embryos generated by mouse embryonic fibroblast (MEF) donor NT and in vivo embryos at each stage (zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst). According to the results from further analyses, NT embryos exhibit RNA processing and translation initiation defects during the zygotic genome activation (ZGA) period, and protein kinase activity and protein phosphorylation are defective during blastocyst formation. Two thousand three constant genes are not able to be reprogrammed in CCs and MEFs. Among these constant genes, 136 genes are continuously mis-transcribed throughout all developmental stages. These 136 differential genes may be reprogramming barrier genes (RBGs) and more studies are needed to identify.
conclusionsThese embryonic transcriptome blueprints provide new data for further mechanistic studies of somatic nuclear reprogramming. These findings may improve the efficiency of somatic cell nuclear transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.