ArticleCell cycle (Georgetown, Tex.)2018
Physical confinement alters sarcoma cell cycle progression and division.
Article in Cell cycle (Georgetown, Tex.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- A customizable, low-cost 3D-printed device for live cell confinement imaging.Lab on a chip · 2026Article
- A vertically integrated system for tracking and assessing cell-cycle-aware phenotypes under confinement.APL bioengineering · 2026Article
- Article
- Cell-Level Modelling of Homeostasis in Confined Epithelial Monolayers.Journal Of Elasticity · 2025Article
- 3D confinement alters smooth muscle cell responses to chemical and mechanical cues.APL bioengineering · 2024Article
- Compressive stresses in cancer: characterization and implications for tumour progression and treatment.Nature reviews. Cancer · 2024Review
- Coordinated in confined migration: crosstalk between the nucleus and ion channel-mediated mechanosensation.Trends in cell biology · 2024Review
- Modelling and targeting mechanical forces in organ fibrosis.Nature reviews bioengineering · 2024Article
- Downregulation of YAP Activity Restricts P53 Hyperactivation to Promote Cell Survival in Confinement.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Cell-extracellular matrix mechanotransduction in 3D.Nature reviews. Molecular cell biology · 2023Review
- Confined migration induces heterochromatin formation and alters chromatin accessibility.iScience · 2022Article
- Mechanical regulation of cell-cycle progression and division.Trends in cell biology · 2022Review
- Microfluidic Lab-on-a-Chip for Studies of Cell Migration under Spatial Confinement.Biosensors · 2022Review
- Compression enhances invasive phenotype and matrix degradation of breast Cancer cells via Piezo1 activation.BMC molecular and cell biology · 2022Article
- Nuclear Deformation Causes DNA Damage by Increasing Replication Stress.Current biology : CB · 2021Article
- Tissue engineered platforms for studying primary and metastatic neoplasm behavior in bone.Journal of biomechanics · 2021Review
- Adhesion-growth factor crosstalk regulates AURKB activation and ERK signalling in re-adherent fibroblasts.Journal of biosciences · 2021Article
- Embracing Mechanobiology in Next Generation Organ-On-A-Chip Models of Bone Metastasis.Frontiers in medical technology · 2021Review
- Mechanosensing of Mechanical Confinement by Mesenchymal-Like Cells.Frontiers in physiology · 2020Review
- Integration of Mesenchymal Stem Cells into a Novel Micropillar Confinement Assay.Tissue engineering. Part C, Methods · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tumor cells experience physical confinement on one or multiple axes, both in the primary tumor and at multiple stages during metastasis. Recent work has shown that confinement in a 3D spheroid alters nucleus geometry and delays cell division, and that vertical confinement impairs mitotic spindle rounding, resulting in abnormal division events. Meanwhile, the effects of bi-axial confinement on cell cycle progression has received little attention. Given the critical role of nuclear shape and mechanics in cell division, we hypothesized that bi-axial physical confinement of the cell body and nucleus would alter cell cycle progression. We used sarcoma cells stably expressing the fluorescence ubiquitination cell cycle indicator (FUCCI), along with fibronectin-coated microchannel devices, and explored the impact of bi-axial physical confinement on cell cycle progression. Our results demonstrate that bi-axial physical confinement reduces the frequency of cell division, which we found to be attributed to an arrest in the S/G2/M phase of the cell cycle, and increases the frequency of abnormal division events. Cell and nuclear morphology were both altered in confinement, with the most confining channels preventing cells from undergoing the normal increase in size from G1 to S/G2/M during cell cycle progression. Finally, our results suggest that confinement induces a mechanical memory to the cells, given our observation of lasting effects on cell division and morphology, even after cells exited confinement. Together, our results provide new insights into the possible impact of mechanical forces on primary and secondary tumor formation and growth.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.