Evidence map›Paper›PMID 30304039›Full record

ArticlePloS one2018

Time resolved 3D live-cell imaging on implants.

Alexandra Ingendoh-Tsakmakidis, Lena Nolte, Andreas Winkel, Heiko Meyer, Anastasia Koroleva, Anastasia Shpichka, Tammo Ripken, Alexander Heisterkamp, Meike Stiesch

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Alexandra Ingendoh-TsakmakidisDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.ORCID 0000-0002-4665-8022
Lena NolteIndustrial and Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
Andreas WinkelDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.
Heiko MeyerIndustrial and Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.
Anastasia KorolevaNanotechnology Department, Laser Zentrum Hannover e.V., Hannover, Germany.
Anastasia ShpichkaInstitute for Regenerative Medicine, Sechenov First Moscow State Medical University, Moscow, Russia.ORCID 0000-0002-9918-9979
Tammo RipkenIndustrial and Biomedical Optics Department, Laser Zentrum Hannover e.V., Hannover, Germany.ORCID 0000-0003-3512-9544
Alexander HeisterkampInstitute of Quantum Optics, Leibniz University of Hanover, Hannover, Germany.
Meike StieschDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Hannover, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials are developed in order to minimize the risk of peri-implant inflammations. The broad range of material testing is conducted using standard 2D, terminal, and invasive methods. The methods that have been applied are not sufficient to monitor the whole implant surface and temporal progress. Therefore, we built a 3D peri-implant model using a cylindrical implant colonized by human gingival fibroblasts. In order to monitor the cell response over time, a non-toxic LIVE/DEAD staining was established and applied to the new 3D model. Our LIVE/DEAD staining method in combination with the time resolved 3D visualization using Scanning Laser Optical Tomography (SLOT), allowed us to monitor the cell death path along the implant in the 3D peri-implant model. The differentiation of living and dead gingival fibroblasts in response to toxicity was effectively supported by the LIVE/DEAD staining. Furthermore, it was possible to visualize the whole cell-colonized implant in 3D and up to 63 hours. This new methodology offers the opportunity to record the long-term cell response on external stress factors, along the dental implant and thus to evaluate the performance of novel materials/surfaces.

Indexed as

Culture MediaDental ImplantsDental Stress AnalysisFibrinFibroblastsGingivaHumansImaging, Three-DimensionalPolyethylene GlycolsPrimary Cell CultureStaining and LabelingTime FactorsTitaniumTomography, Optical CoherenceCulture MediaDental ImplantsFibrinPolyethylene GlycolsTitanium

Identifiers

PMID30304039
PMCPMC6179276

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.