ArticlePLoS neglected tropical diseases2018
Transcriptomic and functional analyses of the piRNA pathway in the Chagas disease vector Rhodnius prolixus.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Article
- Non-gonadal expression of piRNAs is widespread across Arthropoda.FEBS letters · 2025Review
- Embryonic piRNAs target horizontally transferred vertebrate transposons in assassin bugs.Frontiers in cell and developmental biology · 2024Article
- ACurrent research in insect science · 2024Article
- A pro-BMP function exerted byOpen biology · 2023Article
- Review
- A fat body transcriptome analysis of the immune responses of Rhodnius prolixus to artificial infections with bacteria.Parasites & vectors · 2022Article
- Atypical strategies for cuticle pigmentation in the blood-feeding hemipteran Rhodnius prolixus.Genetics · 2022Article
- Dynamics of maternal gene expression in Rhodnius prolixus.Scientific reports · 2022Article
- Transovarial transmission of a core virome in the Chagas disease vector Rhodnius prolixus.PLoS pathogens · 2021Article
- Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus.Development genes and evolution · 2021Article
- Analysis of ovarian transcriptomes reveals thousands of novel genes in the insect vector Rhodnius prolixus.Scientific reports · 2021Article
- Next Generation dsRNA-Based Insect Control: Success So Far and Challenges.Frontiers in plant science · 2021Review
- Exploiting somatic piRNAs inLife science alliance · 2020Article
- RNAi-Based Functional Genomics in Hemiptera.Insects · 2020Review
- Transcriptomic analysis of regulatory pathways involved in female reproductive physiology of Rhodnius prolixus under different nutritional states.Scientific reports · 2020Article
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8 authors at 3 institutions in 1 country.
Funding
Abstract
The piRNA pathway is a surveillance system that guarantees oogenesis and adult fertility in a range of animal species. The pathway is centered on PIWI clade Argonaute proteins and the associated small non-coding RNAs termed piRNAs. In this study, we set to investigate the evolutionary conservation of the piRNA pathway in the hemimetabolous insect Rhodnius prolixus. Our transcriptome profiling reveals that core components of the pathway are expressed during previtellogenic stages of oogenesis. Rhodnius' genome harbors four putative piwi orthologs. We show that Rp-piwi2, Rp-piwi3 and Rp-ago3, but not Rp-piwi1 transcripts are produced in the germline tissues and maternally deposited in the mature eggs. Consistent with a role in Rhodnius oogenesis, parental RNAi against the Rp-piwi2, Rp-piwi3 and Rp-ago3 results in severe egg laying and female adult fertility defects. Furthermore, we show that the reduction of the Rp-piwi2 levels by parental RNAi disrupts oogenesis by causing a dramatic loss of trophocytes, egg chamber degeneration and oogenesis arrest. Intriguingly, the putative Rp-Piwi2 protein features a polyglutamine tract at its N-terminal region, which is conserved in PIWI proteins encoded in the genome of other Triatomine species. Together with R. prolixus, these hematophagous insects are primary vectors of the Chagas disease. Thus, our data shed more light on the evolution of the piRNA pathway and provide a framework for the development of new control strategies for Chagas disease insect vectors.
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