Evidence map›Paper›PMID 30302745›Full record

ArticleHistology and histopathology2019

Membrane trafficking and exocytosis are upregulated in port wine stain blood vessels.

Rong Yin, Shawn J Rice, Jinwei Wang, Lin Gao, Joseph Tsai, Radean T Anvari, Fang Zhou, Xin Liu, Gang Wang, Yuxin Tang and 5 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Histology and histopathology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Pathogenesis of Port-Wine Stains: Directions for Future Therapies.International journal of molecular sciences · 2022
    Review
  4. Article
  5. Review
  6. Observational
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 2 countries.

Rong YinDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Shawn J RicePenn State Cancer Institute, Hershey, PA, USA.
Jinwei WangDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Lin GaoDepartment of Dermatology, Xijing Hospital, Xi'an, China.
Joseph TsaiDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Radean T AnvariDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Fang ZhouDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Xin LiuPenn State Cancer Institute, Hershey, PA, USA.
Gang WangDepartment of Dermatology, Xijing Hospital, Xi'an, China.
Yuxin TangDepartment of Urology, the Xiangya 3rd Hospital, Xiangya School of Medicine, Central South University, Changsha, Hunan, China.
Martin C MihmDepartment of Dermatology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Chandra P BelaniPenn State Cancer Institute, Hershey, PA, USA.
Dong-Bao ChenDepartment of Obstetrics and Gynecology, University of California, Irvine, California, USA.
J Stuart NelsonDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Wenbin TanDepartment of Surgery, Beckman Laser Institute and Medical Clinic, University of California, Irvine, California, USA.
Penn State Milton S. Hershey Medical Center · USUniversity of California, Irvine · USXijing Hospital · CNHarvard University · USUniversity of South Carolina · US

Funding

University of California Health Participation in the National COVID Cohort Collaborative (N3C)UL1TR001414 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI COOPER, DAN M, VILAIN, ERIC J. · 2015 to 2023
$35.1M
Actions of Estrogen on Uterine Artery EndotheliumR01HL070562 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHEN, DONGBAO · 2001 to 2025
$6.3M
UCI P30 Skin Center Systems Biology CoreP30AR075047 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI LANDER, ARTHUR D · 2019 to 2025
$5.1M
Vascular Pathogenesis of Port Wine StainR01AR073172 · NIAMS · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI TAN, WENBIN · 2018 to 2022
$1.5M
Novel Approach to Improve Port Wine Stain Treatment in Human SkinR01AR059244 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NELSON, JOHN STUART · 2010 to 2012
$930k
Mid-Career Investigator in Patient-Oriented ResearchK24AR047551 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NELSON, JOHN STUART · 2003 to 2007
$655k
Molecular mechanisms of pulsed dye laser combined with topical rapamycin for portK01AR063766 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI TAN, WENBIN · 2013 to 2017
$444k
NCATS NIH HHS UL1 TR001414NHLBI NIH HHS R01 HL070562NIAMS NIH HHS K01 AR063766NIAMS NIH HHS K24 AR047551NIAMS NIH HHS P30 AR075047NIAMS NIH HHS R01 AR059244NIAMS NIH HHS R01 AR073172NIH HHS AR063766NIH HHS AR073172NIH HHS AR47551NIH HHS AR59244NIH HHS HL70562
6 · The paper itself

Abstract

introductionPort wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS.

methodsSequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM).

results107 out of 299 identified proteins showed differential expressions in PWS lesions as compared to normal skin, mainly involving the functions of biosynthesis, membrane trafficking, cytoskeleton and cell adhesion/migration. The confirmative studies showed that expressions of membrane trafficking/exocytosis related proteins such as VAT1, IQGAP1, HSC70, clathrin, perlecan, spectrin α1 and GDIR1 were significantly increased in PWS blood vessels as compared to normal ones; while collagen subtypes 6A1 and 6A3 were decreased in PWS skin. Furthermore, TEM studies showed there is a significant upregulation of extracellular vesicle exocytosis from PWS blood vessels as compared to control.

conclusionsThe biological process of membrane trafficking and exocytosis is enhanced in PWS blood vessels. Our results imply that the extracellular vesicles released by lesional endothelial cells may act as potential intercellular signaling mediators to contribute to the pathogenesis of PWS.

Indexed as

Endothelial CellsExocytosisHumansPort-Wine StainProtein TransportUp-Regulation

Identifiers

PMID30302745
PMCPMC6594380
OpenAlexW2946622743

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.