ArticleMikrochimica acta2018
A voltammetric assay for microRNA-25 based on the use of amino-functionalized graphene quantum dots and ss- and ds-DNAs as gene probes.
Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed.
- Design of a lateral flow assay targeting the conserved NIID_2019-nCoV_N gene region for molecular viral diagnosis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025Article
- Article
- Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review.ACS omega · 2022Review
- Two-Dimensional Quantum Dot-Based Electrochemical Biosensors.Biosensors · 2022Review
- Recent Progress in Nanomaterials Modified Electrochemical Biosensors for the Detection of MicroRNA.Micromachines · 2021Review
- Electrochemiluminescence biosensor for microRNA determination based on AgNCs@MoSMikrochimica acta · 2021Article
- Experimental and theoretical study for miR-155 detection through resveratrol interaction with nucleic acids using magnetic core-shell nanoparticles.Mikrochimica acta · 2020Article
- Applications of Graphene Quantum Dots in Biomedical Sensors.Sensors (Basel, Switzerland) · 2020Review
- Article
- Biomarkers-based Biosensing and Bioimaging with Graphene for Cancer Diagnosis.Nanomaterials (Basel, Switzerland) · 2019Review
- Graphene oxide with in-situ grown Prussian Blue as an electrochemical probe for microRNA-122.Mikrochimica acta · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The authors describe a DNA based voltammetric assay for the cancer biomarker microRNA-25. A glassy carbon electrode (GCE) was modified with amino-functionalized graphene quantum dots and used as an amplifier of electrochemical signals. p-Biphenol is introduced as a new electroactive probe with a fairly low working potential of 0.3 V (vs. Ag/AgCl). The stages of fabricating the electrode were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. ss-Probe DNA was immobilized on the modified GCE and then exposed to a sample containing microRNA-25. The results indicated that the electrode can distinguish complementary microRNA-25 from a single-base mismatch. The increase in the electrochemical response of PBP and the positive shift in the potential peak indicate that PBP is intercalated between two strands. Under optimized experimental conditions, the current of the electrode increases linearly with the logarithm of the microRNA-25 concentration in the range from 0.3 nM to 1.0 μM, and the detection limit is 95.0 pM. The assay was successfully employed to the determination of microRNA-25 in spiked human plasma. Graphical abstract A novel electrochemical nanogenosensor is introduced for simple and sensitive determination of microRNA-25, as a biomarker, based on amino-functionalized graphene quantum dots (as a surface modifier) and p-biphenol (as an electroactive label).
Indexed as
Identifiers
30302568What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.