ArticleLight, science & applications2018
Attenuation artifacts in light sheet fluorescence microscopy corrected by OPTiSPIM.
Article in Light, science & applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Metalens-Based Dual Light-Sheet Fluorescence Microscopy.Small methods · 2025Article
- Cytoskeleton imaging of colorectal and lung cancer spheroids using light sheet microscopy.BJC reports · 2025Article
- Deep learning enhanced light sheet fluorescence microscopy for in vivo 4D imaging of zebrafish heart beating.Light, science & applications · 2025Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Light-sheet mesoscopy with the Mesolens provides fast sub-cellular resolution imaging throughout large tissue volumes.iScience · 2022Article
- Engineering a better light sheet in an axicon-based system using a flattened Gaussian beam of low order.Journal of biophotonics · 2022Article
- Benchmarking of deep learning algorithms for 3D instance segmentation of confocal image datasets.PLoS computational biology · 2022Review
- Segmentation of Tissues and Proliferating Cells in Light-Sheet Microscopy Images of Mouse Embryos Using Convolutional Neural Networks.IEEE access : practical innovations, open solutions · 2022Article
- High resolution optical projection tomography platform for multispectral imaging of the mouse gut.Biomedical optics express · 2021Article
- Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction.Scientific reports · 2020Article
- Article
- Fast multi-directional DSLM for confocal detection without striping artifacts.Biomedical optics express · 2020Article
- Applications, promises, and pitfalls of deep learning for fluorescence image reconstruction.Nature methods · 2019Review
- Spatially modulated illumination allows for light sheet fluorescence microscopy with an incoherent source and compressive sensing.Biomedical optics express · 2019Article
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Authors and funding
4 authors.
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Abstract
Light sheet fluorescence microscopy (LSFM) is rapidly becoming an essential technology for mesoscopic imaging of samples such as embryos and adult mouse organs. However, LSFM can suffer from optical artifacts for which there is no intrinsic solution. The attenuation of light due to absorbing material causes "shadow" artifacts along both the illumination and detection paths. Several approaches have been introduced to reduce this problem, including scanning illumination and multi-view imaging. However, neither of these approaches completely eliminates the problem. If the distribution of the absorbing material is complex, shadows cannot be avoided. We introduce a new approach that relies on multi-modal integration of two very different mesoscopic techniques. Unlike LSFM, optical projection tomography (OPT) can operate in transmission mode to create a voxel map of the 3D distribution of the sample's optical attenuation. Here, we demonstrate a hybrid instrument (OPTiSPIM) that can quantify this attenuation and use the information to correct the shadow artifacts of LSFM.
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Registered trials
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